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棉花学报  2011 

市售草甘膦除草剂对转基因抗草甘膦棉花幼苗生长的影响

DOI: 1002-7807(2011)05-0408-06, PP. 408-413

Keywords: 棉花,EPSPS-G6,POD活性,可溶性蛋白,草甘膦

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Abstract:

以转基因抗草甘膦棉花种质系G6-7和G6-8及其非转基因遗传背景亲本对照中棉所49为材料,研究了市售10%草甘膦水剂和95%的草甘膦粉剂对抗草甘膦棉花种子萌发和幼苗生长的影响。结果表明,中棉所49对于两种类型的草甘膦均很敏感,幼苗喷施0.2mmol·L-1的10%草甘膦水剂和95%草甘膦粉剂均全部死亡。转EPSPS-G6基因抗草甘膦棉对95%草甘膦粉剂的抗性较好,但对10%草甘膦水剂的抗性较弱。就转基因棉花对两种剂型草甘膦的反应看,喷施2.4mmol·L-1的10%草甘膦水剂和95%草甘膦粉剂后,棉花幼苗全株鲜重、叶重、根重、下胚轴长、可溶性蛋白质增量和POD活性等各项指标在两种草甘膦制剂间的差异均达到显著水平。喷施12.0mmol·L-1的10%草甘膦水剂,转基因抗草甘膦棉花的种子发芽率显著低于相同浓度的粉剂,两者之间的发芽率相差25%~75%。研究结果表明,10%的草甘膦水剂中可能含有对棉花幼苗生长发育有害的物质,影响抗草甘膦棉幼苗的生长,在转基因抗草甘膦棉花品种商业化应用时应谨慎使用。

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