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棉花学报  2014 

陆地棉SSR标记遗传多样性及其与农艺性状的关联分析

DOI: 1002-7807(2014)05-0420-11, PP. 420-430

Keywords: 陆地棉,关联分析,SSR,遗传多样性,群体结构

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Abstract:

分析陆地棉栽培种遗传多样性,通过关联分析寻找与棉花农艺性状相关联的分子标记,为分子标记辅助选择育种和提高棉花育种效率奠定基础。本文采用74个Simplesequencerepeat(SSR)标记对172份陆地棉栽培种的基因组变异进行扫描,使用NTSYS-pc2.20进行聚类,分析该群体遗传多样性;利用Structure2.3.4软件分析群体结构,在此基础上结合田间表型数据,采用Tassel2.1的一般线性模型(Generallinearmodel,GLM)进行关联分析,定位与农艺性状相关的QTLs。74个标记共检测到148个多态性位点,涉及246个等位变异,变异范围2~7个,平均等位变异数为3.32;引物的多态性信息含量(PIC)为0.0281~0.3733,平均值为0.2370;遗传相似系数变异在0.2816~1,平均值为0.5369,平均遗传相似系数为0.5369,表明我国陆地棉遗传基础狭窄,尽管国外及西北内陆棉区部分材料具有较丰富的遗传变异。聚类分析将该群体划分为12个亚群,不同棉区的材料交叉分布,且聚类结果基本与系谱吻合。群体结构分析却将172份供试材料划分为3个亚群;通过关联分析,发现30个位点与铃重、衣分、黄萎病抗性显著相关(P<0.05),各位点对表型变异贡献率为2.24%~5.27%。

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