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棉花学报  2014 

棉花盐胁迫下叶片miRNAs的鉴定与分析

DOI: 1002-7807(2014)05-0377-10, PP. 377-386

Keywords: 盐胁迫,miRNA,小RNA测序,靶基因预测

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Abstract:

microRNAs(miRNAs)是一类21~24个核酸长度的非编码小分子RNA(sRNA,smallRNA),通过介导目标mRNA的切割或者抑制翻译来调节植物基因的表达。本文利用200mmol·L-1NaCl分别处理3叶期耐盐品系早熟长绒7号和盐敏感品系南丹巴地大花4h,构建了4个小RNA文库并测序。以雷蒙德氏棉(GossypiumraimondiiL.)D5基因组和本实验室陆地棉(GossypiumhirsutumL.)盐胁迫转录组测序拼接得到的序列作为参考,共发现360个miRNA,包括308个保守的miRNA和52个可信度高的新miRNA。2个品系共出现94个差异表达的miRNA,表达模式可分为2大类,筛选出20个候选miRNA。KEGG(Kyotoencyclopediaofgenesandgenomes)分析表明,耐盐品系显著富集的信号通路是抗原加工过程,而盐敏感品系显著富集的是生物碱合成途径。

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