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棉花学报  2015 

大草蛉化学感受蛋白基因CpalCSP3组织表达谱及气味结合特性分析

DOI: 10.11963/issn.1002-7807.201503010, PP. 260-267

Keywords: 大草蛉,化学感受蛋白,组织表达谱,原核表达|荧光竞争结合试验

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Abstract:

昆虫化学感受蛋白(Chemosensoryprotein,CSP)具有结合和运载气味分子穿过感器淋巴液到达嗅觉受体的作用,但尚未见有关大草蛉(Chrysopapallens)CSP功能的报道。本研究克隆得到大草蛉CpalCSP3基因,利用RT-qPCR技术测定了其在成虫各组织的表达谱,发现CpalCSP3基因主要在雌雄虫的触角和头部表达,暗示该基因参与了大草蛉的嗅觉及味觉感受。为探讨其嗅觉功能,利用原核表达系统和荧光竞争结合试验测定了CpalCSP3蛋白与84种气味物质的结合能力。结果表明,CpalCSP3蛋白与橙花叔醇、(+) ̄雪松醇和β ̄紫罗兰酮具有很高的结合能力,结合常数(Ki)为8.79~9.79μmol·L-1;与根皮苷、月桂醛、乙酸香叶酯和α ̄蒎烯也有较高的结合能力,Ki为11.54~19.06μmol·L-1。分析认为,CpalCSP3蛋白可能在大草蛉对这些气味的嗅觉感受中起作用,使得大草蛉能对害虫寄主植物有效定向进而搜寻到猎物。

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