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棉花学报  2015 

棉花C2H2类型锌指蛋白基因GhSIZ1的克隆及表达分析

DOI: 10.11963/issn.1002-7807.201503001, PP. 189-197

Keywords: 棉花,C2H2型锌指蛋白,表达模式,GhSIZ1

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Abstract:

通过RT-PCR技术从陆地棉中克隆到1个编码C2H2型锌指蛋白的基因,命名为GhSIZ1(StressInducedZincfingerprotein1)。该基因编码239个氨基酸残基,预测蛋白分子量为26.6205kD,等电点为6.52。GhSIZ1包含一个C2H2型锌指结构域,在其N端含有保守的L-box,C–末端含有一个转录抑制结构域EAR/DLN-box。系统发育树分析表明GhSIZ1与可可(XP_007044496.1)和中粒咖啡(CDP00218.1)中的锌指蛋白亲缘关系较近。实时定量PCR分析GhSIZ1在棉花各组织中均有表达,但在根中表达量最高。低温、高盐、干旱胁迫处理后GhSIZ1的表达水平显著上调。亚细胞定位分析表明,GhSIZ1定位在细胞核中。本研究表明,GhSIZ1可能在棉花响应低温、高盐、干旱等非生物胁迫中起着重要作用。

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