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棉花学报  2014 

陆地棉单萜合酶基因GhTPS4和GhTPS5的克隆及棉铃虫诱导的基因表达分析

DOI: 1002-7807(2014)06-0513-08, PP. 513-520

Keywords: 棉花,单萜合酶,GhTPS4,GhTPS5,棉铃虫,基因表达

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Abstract:

通过本地Blast筛选陆地棉转录组数据并结合RACE的方法,克隆获得2个陆地棉萜类合酶基因全长,命名为GhTPS4(Genebank登录号KF860865)和GhTPS5(Genebank登录号KF860866)。GhTPS4和GhTPS5基因分别编码590个和598个氨基酸,预测分子量为68.5kDa和69.9kDa,等电点为5.59和5.66。生物信息分析表明GhTPS4和GhTPS5均含有叶绿体转运肽,并含有“DDxxD”和“RRX8W”保守域,具有典型的单萜合酶特征。此外,聚类分析表明GhTPS4和GhTPS5均属于Tpsb亚家族。利用荧光定量PCR的方法,对GhTPS4和GhTPS5在棉铃虫为害陆地棉(石远321)叶片的表达模式进行了研究,结果表明GhTPS4的表达量在处理后不同时间均高于未处理对照,在处理后36h表达量最高,表明GhTPS4在应答棉铃虫为害时可能起到了积极的防御作用。GhTPS5在处理后不同时间均比未处理对照表达量低,其功能尚需进一步探讨。

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