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棉花学报  2015 

棉花转录因子基因GhMYB的克隆及特征分析

DOI: 1002-7807(2015)01-0031-08, PP. 31-38

Keywords: 棉花,GhMYB,原核表达,基因克隆

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Abstract:

从棉花中克隆了1个MYB基因,命名为GhMYB(GenBankNo.HQ234875)。该基因cDNA全长1264bp,具有1个774bp的开放阅读框,5'非编码区为250bp,3'非编码区为240bp,编码256个氨基酸,预测分子量约为28.867kDa,等电点为8.56,推测的氨基酸序列中含有2个高度保守的SANT结构域。GhMYB基因组序列长度为1355bp,包含2个外显子和1个内含子。氨基酸同源分析发现,GhMYB与来自可可、陆地棉和巴旦木的MYB同源性较高。亚细胞定位结果表明,GhMYBGFP融合蛋白定位于细胞核中。SDS-PAGE电泳分析表明,pET-28a-GhMYB最佳诱导表达条件为1.0mmol·L-1IPTG在37℃下诱导4h。电子表达谱分析表明,GhMYB基因在棉铃中特异表达,在根和叶中受水涝胁迫的诱导表达。

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