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棉花学报  2015 

样品制备对转基因抗虫棉外源Bt蛋白定量检测的影响

DOI: 10.11963/issn.1002-7807.201502012, PP. 184-188

Keywords: 转基因抗虫棉,样品制备,Bt蛋白含量,定量检测,ELISA

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Abstract:

采用ELISA（Enzyme-linkedimmunosorbentassay）方法，以转Bt基因抗虫棉品种中棉所45（ZGK9822）和鄂抗虫棉1号（GK19）为研究材料，对叶片的不同组织、同一样品不同的冻存时间和同一样品不同研磨程度的Bt蛋白进行定量检测。结果表明叶片不同组织Bt蛋白表达量差异较小，叶脉Bt蛋白表达量略高于叶肉和叶柄的Bt蛋白表达量；转基因抗虫棉单位鲜物质质量Bt蛋白含量和单位可溶性总蛋白Bt蛋白含量随着冻存时间的延长而降低，鄂抗虫棉1号单位鲜物质质量Bt蛋白含量冻存90d样品与冻存30d、60d样品差异显著，单位可溶性总蛋白Bt蛋白含量无显著差异；不同研磨程度对转基因抗虫棉单位鲜物质质量Bt蛋白含量和单位可溶性总蛋白Bt蛋白含量都存在影响，其中差异最大的为鄂抗虫棉1号，200目试样比50目试样单位鲜物质质量Bt蛋白含量上升了56%，单位可溶性总蛋白Bt蛋白含量上升了38%。

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