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棉花学报  2015 

陆地棉耐盐性状与SSR分子标记的关联分析

DOI: 10.11963/issn.1002-7807.201502004, PP. 118-125

Keywords: 陆地棉,关联分析,SSR分子标记,耐盐性

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Abstract:

本研究以134份陆地棉栽培种为试验材料，测定其在0.3%盐浓度（质量分数）下的出苗率，并使用74对SSR引物对这些材料进行基因组变异扫描。利用Structure2.3.4软件分析该自然群体的遗传结构，在此基础上采用Tassel2.1软件对耐盐性状与SSR分子标记进行关联分析，寻找与棉花耐盐性状相关的分子标记。研究结果表明（1）134份陆地棉栽培种的出苗率呈极显著差异，并筛选出27个盐敏感材料和10个耐盐材料。（2）74个SSR分子标记共检测出148个多态性位点，涉及246个等位变异，变异范围为2～7，平均每个标记3.32个；基因多样性指数变异范围为0.0295～0.4959，平均值为0.2897；SSR分子标记多态性信息含量（PIC）变幅为0.0290～0.3729，平均值为0.2381。（3）通过群体结构分析，将该自然群体划分2个亚群体，分别包含89份和45份材料。（4）关联分析共发现8个与棉花耐盐性状相关的SSR分子标记位点，表型变异解释率变幅为2.91%～7.82%，平均值为4.32%。此研究结果可以为棉花耐盐性状分子标记辅助选择育种提供参考。

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