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棉花学报  2015 

三个栽培种中天然彩色棉的遗传多样性及关联分析

DOI: 10.11963/issn.1002-7807.201504001, PP. 291-299

Keywords: 彩色棉,遗传多样性,关联分析,SSR标记

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Abstract:

本研究利用主要农艺性状和SSR标记分析了137份陆地棉、13份海岛棉以及27份亚洲棉等不同纤维色泽的种质资源。170对SSR引物共检测到1036个等位基因变异。其中有多态性的等位基因923个,平均每个SSR位点有5.43个等位变异,变化范围为1~10。位点多态性信息量(PIC)的变化范围为0.56~0.93,PIC在0.85以上的多态性SSR位点为122个。结果表明,实验材料存在着丰富的遗传多样性。在群体结构分析基础上,使用TASSEL软件中的混合线性模型(MLM),在陆地棉中得到与11个性状相关联的SSR标记23个;在海岛棉群体得到与5个性状关联的17个标记;在亚洲棉中检测到与8个性状关联的15个标记位点。在三个栽培种中与纤维色泽度相关的标记位点一共有6个,分别是CIR51-4、BNL1421-2、BNL2656-2、DPL570-2、GH268-6和TMB131-1,表型变异解释率从3.12%到18.97%。三个栽培种中天然彩色棉种质具有丰富的遗传多样性,为棉花的种间、种内杂交育种以及拓宽棉花新品种的遗传背景提供了物质基础和理论依据。

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