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棉花学报  2015 

光质对离体培养条件下绿色棉纤维发育及黄酮合成的影响

DOI: 10.11963/issn.1002-7807.201504002, PP. 300-309

Keywords: 绿色棉,离体培养,光质,基因表达,纤维发育,黄酮合成

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Abstract:

为探讨光质对绿色棉纤维生长发育和黄酮合成的影响,以绿色棉品种绿絮棉1号为材料进行离体培养,比较红光、黄光、蓝紫光、白光和暗处理条件下绿色棉胚珠鲜物质质量、纤维长度、纤维素含量、黄酮含量等生理和分子指标的差异。结果表明光处理能够促进胚珠鲜物质质量的增加,但抑制了纤维的伸长。在纤维发育前期,各光处理抑制了蔗糖合成酶活性和蔗糖合成酶基因表达;纤维发育后期,白光处理提高了蔗糖合成酶活性,促进了蔗糖合成酶基因表达。红光、黄光和蓝紫光处理显著提高了β-1,3 ̄葡聚糖酶活性(10~20DAC(Daysafterculture));红光、黄光和白光均能提高β-1,3 ̄葡聚糖基因表达量,而蓝光则抑制了基因的表达。白光促进了纤维素的合成,而蓝紫光抑制了纤维素的合成。在纤维发育初期(10DAC),蓝紫光抑制了黄酮合成及其关键酶基因表达;而在发育后期(30~40DAC),白光处理能够促进黄酮的合成及其关键酶基因表达。可见,光质对绿色棉纤维发育及黄酮合成具有一定的影响。

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