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科学通报  2011 

水稻早衰叶突变体es-t的遗传分析与精细定位

, PP. 1539-1545

Keywords: 水稻,早衰突变体es-t,基因定位

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Abstract:

突变体es-t是经EMS诱变处理日本晴后筛选获得的,该突变体主要表现为叶片从苗期开始黄化,叶绿素含量显著降低,随着其生长发育发黄的叶片伴有铁锈色的小斑点,尤以叶尖和叶缘为甚,表现严重的早衰现象,故将之命名为es-t(earlysenescence-temporary).扫描电子显微镜显示,突变体叶片表面比野生型的光滑,且气孔周围缺乏硅质化突起;另外,突变体的叶绿体发育不正常,含有大量大颗粒的淀粉粒;组织切片则显示突变体的厚壁细胞及维管束的发育表现异常.遗传分析表明,es-t为新发现的早衰突变体,受一隐性基因控制,借助图位克隆的手段将之定位于42.1kb的物理区间内,为进一步克隆该基因并阐明叶片早衰的分子机制奠定基础.

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