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科学通报  2011 

CdTe/ZnS量子点荧光可逆调控研究ctDNA与吡柔比星的相互作用

DOI: 10.1360/972010-2275, PP. 1804-1811

Keywords: CdTe/ZnS,量子点,吡柔比星,ctDNA,荧光

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Abstract:

水相合成了谷胱甘肽修饰的CdTe/ZnS量子点(GSH-CdTe/ZnSQDs).从原子力显微镜照片可以看出,合成的核壳型GSH-CdTe/ZnSQDs具有良好的分散性.当吡柔比星与GSH-CdTe/ZnSQDs相互作用时,吡柔比星吸附在GSH-CdTe/ZnSQDs的表面,通过光诱导电子转移的方式猝灭GSH-CdTe/ZnSQDs的荧光.然后向GSH-CdTe/ZnSQDs-吡柔比星体系中加入ctDNA,吡柔比星从GSH-CdTe/ZnSQDs表面脱落后嵌入ctDNA双螺旋结构中,光诱导电子转移过程被阻断,GSH-CdTe/ZnSQDs的荧光恢复.根据GSH-CdTe/ZnSQDs荧光的猝灭和恢复,实现了量子点荧光的可逆调控.结合共振瑞利散射和紫外吸收光谱,讨论了GSH-CdTe/ZnSQDs吡柔比星-ctDNA的相互作用机理,建立了一种研究蒽醌类抗癌药物与核酸相互作用的光谱方法.

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