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科学通报  2011 

扬子鳄肝脏、心脏和肌肉细胞系构建

, PP. 1780-1783

Keywords: 扬子鳄,保护,组织培养,细胞系

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Abstract:

扬子鳄属国家一级保护的珍稀濒危动物.细胞系的构建和保存是濒危野生动物离体保护工程的主要内容之一.本研究构建了扬子鳄肝脏、心脏和肌肉细胞系.结果表明,从3种不同来源组织块中原代细胞的生长速度表现出明显差异,分别为11~12,13~14和17~18d;而传代细胞的生长速度在3种组织来源的细胞之间没有明显差异,均在6~7d,细胞倍增时间为36h.扬子鳄的染色体核型为2n=32,无明显的性染色体.该研究所建立的3种不同组织来源的细胞系将为扬子鳄的相关保护及研究工作提供可靠的材料平台,也将为其他两栖爬行动物的细胞系构建提供有益参考.

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