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科学通报  2014 

镱对大鼠海马神经元钠电流及其动力学的影响

DOI: 10.1360/csb2014-59-2-210, PP. 210-214

Keywords: 海马神经元,钠通道,镱离子,膜片钳技术

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Abstract:

利用全细胞膜片钳技术,研究稀土镱离子对大鼠海马神经元电压门控性钠电流及其动力学的影响.膜片钳记录到的钠电流经过滤波过滤后储存于计算机中.结果发现,稀土镱离子对海马神经元钠电流的增大效应具有剂量依赖性和电压依赖性.100mmol/LYb3+使电压门控性钠电流的最大电流值由2154±163pA增大到2807±60pA,电流幅度增加了30.3%,这与镱抑制钾电流作用完全不同.镱作用钠电流的半数增大浓度是8.97mmol/L.通过对钠通道激活和失活动力学分析发现,100mmol/LYb3+基本不改变钠通道的激活和失活的电压过程.从激活时间和失活时间常数来分析,它们都具有电压依赖性,在-80和-70mV电压下,100mmol/LYb3+显著延长了钠电流的激活时间,大于-70mV以后,镱不改变峰值时间,Yb3+使钠电流失活时间常数显著减小.总体来说,在钠电流开放的电压范围内,Yb3+基本不影响钠电流的激活过程,使失活时程提前,可以看出Yb3+主要通过增大钠通道的电导而增大钠电流的幅度,这可能是Yb3+作用海马神经元的机制之一.

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