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科学通报  2011 

[Hg(SCN)4]2-与蛋白质相互作用的共振瑞利散射光谱及其分析应用

DOI: 10.1360/972010-1451, PP. 396-406

Keywords: 蛋白质,[Hg(SCN)4]2-,共振瑞利散射,吸收光谱,荧光猝灭

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Abstract:

在pH1.0~4.5的稀硫酸介质中,[Hg(SCN)4]2-配阴离子与人血清白蛋白(HSA)、α-糜蛋白酶(α-Chy)、牛血清白蛋白(BSA)、溶菌酶(Lyso)和γ-球蛋白(γ-G)等蛋白质反应形成复合物,此时将导致共振瑞利散射(RRS)的显著增强,也能引起吸收光谱的变化和荧光猝灭,同时还观察到圆二色(CD)光谱特征的改变.本文主要研究[Hg(SCN)4]2-蛋白质复合物的RRS光谱特征、适宜的反应条件和影响因素,以及分析化学性质,并以[Hg(SCN)4]2-Lyso体系为例,结合吸收、荧光和圆二色光谱的变化,对复合物的结合位点、结合模式以及散射增强的原因进行了讨论.RRS法具有较高的灵敏度,它对不同蛋白质的检出限在4.6~10.8ng/mL之间,据此建立了以[Hg(SCN)4]2-配阴离子作探针测定人血液和尿液中蛋白质的新方法.

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