全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
科学通报  2014 

基于分子印迹物薄膜的血红蛋白表面等离子共振传感器制备与检测

DOI: 10.1360/972013-899, PP. 779-786

Keywords: 分子印迹聚合物膜,表面等离子共振,血红蛋白,3-氨基苯硼酸,自组装

Full-Text   Cite this paper   Add to My Lib

Abstract:

以3-氨基苯硼酸为单体,通过化学沉积的方法在L-半胱氨酸修饰过的金膜表面合成了针对血红蛋白(hemoglobin,Hb)的分子印迹聚合物膜(molecularlyimprintedpolymerfilm,MIF),并作为表面等离子共振传感器(SPR)的识别单元.优化模板Hb同单体的比例,并对该条件下制备的MIF表面形貌及厚度进行表征.优化MIF吸附条件,实验结果表明MIF修饰的SPR传感器对Hb具有较高的灵敏度和吸附能力,该条件下所能检测到的Hb最低浓度值为0.005mg/mL.选择性和稳定性实验结果表明,MIF对牛血清蛋白、溶菌酶和卵清蛋白具有良好的选择效果,同时具有良好的稳定性.尿液配制Hb样品的检测结果显示该传感器在较为复杂的环境中对Hb仍具有良好的响应.

References

[1]  7 Bonini F, Piletsky S, Turner A P F, et al. Surface imprinted beads for the recognition of human serum albumin. Biosens Bioelectron, 2007, 22: 2322-2328
[2]  13 Feng X, Gao F, Qin P, et al. Real time monitoring of on-chip coenzyme regeneration with SPR and DPI. Anal Chem, 2013, 85: 2370-2376
[3]  16 Matsunaqa T, Hishiya T, Takeuchi T. Surface plasmon resonance sensor for lysozyme based on molecularly imprinted thin films. Anal Chim Acta, 2007, 591: 63-67
[4]  17 Gupta G, Singh P K, Boopathi M, et al. Molecularly imprinted polymer for the recognition of biological warfare agent staphylococcal enterotoxin B based on surface plasmon resonance. Thin Solid Films, 2010, 519: 1115-1121
[5]  18 Bao H, Wei T X, Li X L, et al. Detection of TNT by a molecularly imprinted polymer film-based surface plasmon resonance sensor. Chin Sci Bull, 2012, 57: 2102-2105
[6]  19 Wei Q Q, Wei T X. A novel method to prepared SPR sensor chips based on photografting molecularly imprinted polymer. Chin Chem Lett, 2011, 22: 721-724
[7]  20 Turner N W, Liu X, Piletsky S A, et al. Recognition of conformational changes in b-lactoglobulin by molecularly imprinted thin film. Biomacromolecules, 2007, 8: 2781-2787
[8]  21 傅崇岗, 苏昌华, 单瑞峰. L-半胱氨酸自组装膜修饰金电极对抗坏血酸的电催化作用及其测定. 分析化学, 2004, 32: 1349-1352
[9]  22 Piletsky S A, Piletska E V, Bossi A, et al. Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays. Biosens Bioelectron, 2001, 16: 701-707
[10]  24 程鹏, 胡志雄, 张维农, 等. 聚间氨基苯硼酸纳米纤维的化学氧化合成及表征. 武汉工业学院院报, 2013, 32: 48-52
[11]  29 Gobi K V, Kim S J, Tanaka H, et al. Novel surface plasmon resonance (SPR) immunosensor based on monomolecular layer of physically- adsorbed ovalbumin conjugate for detection of 2,4-dichlorophenoxyacetic acid and atomic force microscopy study. Sens Actuators B Chem, 2007, 123: 583-593
[12]  1 Hoppe C C. Prenatal and newborn screening for hemoglobinopathies. Int J Lab Hematol, 2013, 35: 297-305
[13]  2 James T D, Sandanayake K R, Shinkai S. Saccharide sensing with molecular receptors based on boronic acid. Angew Chem Int Ed, 1996, 35: 1910-1922
[14]  3 Asher S A, Alexeev V L, Goponenko A V, et al. Photonic crystal carbohydrate sensors: Low ionic strength sugar sensing. J Am Chem Soc, 2003, 125: 3322-3329
[15]  4 Li L, Lu Y, Bie Z, et al. Photolithographic boronate affinity molecular imprinting: A general and facile approach for glycoprotein imprinting. Angew Chem Int Ed, 2013, 52: 7451-7454
[16]  5 Lu Y, Yan C L, Gao S Y. Preparation and recognition of surface molecularly imprinted core-shell microbeads for protein in aqueous solutions. Appl Surf Sci, 2009, 255: 6061-6066
[17]  6 Wang H, Li W, He X, et al. M-aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin. React Funct Polym, 2008, 68: 1291-1296
[18]  8 Bossi A, Piletsky S A, Piletska E V, et al. Surface-grafted molecularly imprinted polymers for protein recognition. Anal Chem, 2001, 73: 5281-5286
[19]  9 Lee M H, Thomas J L, Tseng H Y, et al. Sensing of digestive proteins in saliva with a molecularly imprinted poly(ethylene-co-viny alcohol) thin film coated quartz crystal microbalance sensor. Appl Mater Interfaces, 2011, 3: 3064-3071
[20]  10 Reddy S M, Phan Q T, Ei-Sharif H, et al. Protein crystallization and biosensor applications of hydrogel-based molecularly imprinted polymers. Biomacromolecules, 2012, 13: 3959-3965
[21]  11 Wang Y, Huang C J, Jonas U, et al. Biosensor based on hydrogel optical waveguide spectroscopy. Biosens Bioelectron, 2010, 25: 1663-1668
[22]  12 Szunerits S, Boukherroub R. Sensing using localised surface plasmon resonance sensors. Chem Commun, 2012, 48: 8999-9010
[23]  14 Hook A L, Thissen H, Voelcker N H. Surface plasmon resonance imaging of polymer microarrays to study protein-polymer interactions in high throughput. Langmuir, 2009, 25: 9173-9181
[24]  15 Corgier B P, Bellon S, Anger-Leroy M, et al. Protein-diazonium adduct direct electrografting onto SPRi-biochip. Langmuir, 2009, 25: 9619-9623
[25]  23 Sun X Y, Liu B, Jiang Y B. An extremely sensitive monoboronic acid based fluorescent sensor for glucose. Anal Chim Acta, 2004, 515: 285-290
[26]  25 Rick J, Chou T C. Enthalpy changes associated with protein binding to thin films. Biosens Bioelectron, 2005, 20: 1878-1883
[27]  26 Ouyang R, Lei J, Ju H. Artificial receptor-functionalized nanoshell: Facile preparation, fast separation and specific protein recognition. Nanotechnology, 2010, 21: 1-9
[28]  27 Xia Y, Guo T, Song M, et al. Hemoglobin recognition by imprinting in semi-interpenetrating polymer network hydrogel based on polyacrylamide and chitosan. Biomacromolecules, 2005, 6: 2601-2606
[29]  28 Kan X, Xing Z, Zhu A, et al. Molecularly imprinted polymers based electrochemical sensor for bovine hemoglobin recognition. Sens Actuators B Chem, 2012, 168: 395-401

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133