全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
科学通报  2014 

产黄青霉形态代谢工程研究进展

DOI: 10.1360/972013-1159, PP. 2017-2032

Keywords: 图像处理,诱变,形态突变株,青霉素产量,几丁质合成酶,RNA干扰

Full-Text   Cite this paper   Add to My Lib

Abstract:

丝状真菌广泛应用于工业微生物发酵,其发酵形态的多样性和调控复杂性为业界广泛关注.本文对产黄青霉的形态、调控以及与产量相关性展开研究.通过一种有效菌丝形态图像处理方法,获得菌丝形态特征参数,并应用于产黄青霉的形态动力学分析;以低能离子注入和低温空气等离子体对产黄青霉进行了复合诱变选育,比较产黄青霉形态突变株与原始菌的形态差异,揭示菌丝形态与次级代谢产物合成的相关性;以Ⅲ类几丁质合成酶基因chs4为目的基因,通过RNA干扰构建形态突变株,发现形态突变株菌丝变得粗短、分枝增多,转化株青霉素产量明显高于原始菌,其中PcRNAi1-17和PcRNAi2-1的青霉素产量分别比原始菌提高27%和41%,为产黄青霉形态代谢工程研究奠定了一定的理论基础.

References

[1]  3 Wang Z, Lauwerijssen M J, Yuan J. Combined age and segregated kinetic model for industrial-scale penicillin fed-batch cultivation. Biotechnol Bioproc Eng, 2005, 10: 142-148
[2]  4 Zangirolami T C, Johansen C L, Nielsen J, et al. Simulation of penicillin production in fed-batch cultivations using a morphologically structured model. Biotechnol Bioeng, 1997, 56: 593-604
[3]  5 马世敏, 祁桂玲, 张弘, 等. 青霉菌的菌丝形态对发酵过程的影响. 河北医药, 2006, 28: 768-769
[4]  8 王鹏, 蔡晓春, 郑之明, 等. 菌丝体显微图像分割及特征提取软件. 软著登字第0317455号, 2011
[5]  11 刘会, 桂芳, 赵根海, 等. Penicillium chrysogenum的低温空气等离子体诱变研究. 第二届全国诱变育种新技术研究与应用交流年会, 2012年12月27~29日, 海口
[6]  12 桂芳, 刘会, 王辉, 等. Penicillium chrysogenum的低温空气等离子体诱变研究. 辐射研究与辐射工艺学报, 2011, 29: 290-296
[7]  13 Culp D W, Dodge C L, Miao Y, et al. The chsA gene from Aspergillus nidulans is necessary for maximal conidiation. FEMS Microbiol Lett, 2000, 182: 349-353
[8]  14 Motoyama T, Sudoh M, Horiuchi H, et al. Isolation and characterization of two chitin synthase genes of Rhizopus oligosporus. Biosci Biotechnol Biochem, 1994, 58: 1685-1693
[9]  15 Motoyama T, Fujiwara M, Kojima N, et al. The Aspergillus nidulans genes chsA and chsD encode chitin synthases which have redundant functions in conidia formation. Mol Gen Genet, 1997, 253: 520-528
[10]  16 Specht C A, Liu Y, Robbins P W, et al. The chsD and chsE genes of Aspergillus nidulans and their roles in chitin synthesis. Fungal Genet Biol, 1996, 20: 153-167
[11]  17 Borgia P T, Iartchouk N, Riggle P J, et al. The chsB gene of Aspergillus nidulans is necessary for normal hyphal growth and development. Fungal Genet Biol, 1996, 20: 193-203
[12]  18 Yanai K, Kojima N, Takaya N, et al. Isolation and characterization of two chitin synthase genes from Aspergillus nidulans. Biosci Biotechnol Biochem, 1994, 58: 1828-1835
[13]  19 Horiuchi H, Fujiwara M, Yamashita S, et al. Proliferation of intrahyphal hyphae caused by disruption of csmA, which encodes a class V chitin synthase with a myosin motor-like domain in Aspergillus nidulans. J Bacteriol, 1999, 181: 3721-3729
[14]  20 Bowen A, Chen-Wu J, Momany M, et al. Classification of fungal chitin synthases. Proc Natl Acad Sci USA, 1992, 89: 519-523
[15]  21 Liu H, Wang P, Gong G, et al. Morphology engineering of Penicillium chrysogenum by RNA silencing of chitin synthase gene. Biotechnol Lett, 2013, 35: 423-429
[16]  22 Liu H, Wang P, Hu Y, et al. Construction of an RNAi expression vector and transformation into Penicillium chrysogenum. Ann Microbiol, 2014, 64: 113-120
[17]  23 刘会, 郑之明, 贡国鸿, 等. 几丁质合成酶在产黄青霉形态发生中的功能研究. 第七届中国工业生物技术发展高峰论坛, 2013年5月9~12日, 天津
[18]  24 Liu H, Zheng Z, Wang P, et al. Morphological changes induced by class III chitin synthase gene silencing could enhance penicillin production of Penicillium chrysogenum. Appl Microbiol Biotechnol, 2013, 97: 3363-3372
[19]  25 王辉, 王鹏, 刘会, 等. 青霉素发酵的形态动力学研究. 第六届全国化学工程与生物化工年会, 2010年10月29~31日, 长沙
[20]  1 McIntyre M, Müller C, Dynesen J, et al. Metabolic engineering of the morphology of Aspergillus. In: Nielsen J, Eggeling L, Dynesen J, et al, eds. Metabolic Engineering. Berlin Heidelberg: Springer, 2001. 103-128
[21]  2 Papagianni M. Fungal morphology and metabolite production in submerged mycelial processes. Biotechnol Adv, 2004, 22: 189-259
[22]  6 Thomas C R. Image analysis: Putting filamentous microorganisms in the picture. Trends Biotechnol, 1992, 10: 343-348
[23]  7 王辉, 桂芳, 王鹏, 等. 菌丝空泡形态比例与青霉素合成的关系. 生物加工过程, 2011, 9: 26-29
[24]  9 桂芳, 刘会, 王鹏, 等. 青霉素产生菌的复合诱变选育. 第六届全国化学工程与生物化工年会, 2010年10月29~31日, 长沙
[25]  10 Gui F, Wang H, Wang P, et al. The Mutation breeding and mutagenic effect of air plasma on Penicillium chrysogenum. Plasma Sci Technol, 2012, 14: 297-302
[26]  26 Obst U. Quorum sensing: Bacterial chatting. Anal Bioanal Chem, 2007, 387: 369-370
[27]  27 Müller C, Hansen K, Szabo P, et al. Effect of deletion of chitin synthase genes on mycelial morphology and culture viscosity in Aspergillus oryzae. Biotechnol Bioeng, 2003, 81: 525-534

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133