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科学通报  2014 

大鼠大脑皮质发育过程中FLnI-L1表达谱分析

DOI: 10.1360/N972014-00625, PP. 3370-3377

Keywords: FLnI-L1,转座,高通量测序,脑皮质,神经发育

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Abstract:

基因组中存在大量重复序列,这些重复序列究竟具有何种生物学功能以及通过何种方式发挥功能目前仍然是一个未解之谜.许多重复序列来源于转座元件在基因组中的整合,longinterspersedelements(LINE-1,L1)逆转座子是哺乳动物基因组中非常重要的一类转座元件,由其来源的DNA序列占基因组的17%,并且L1可能通过多种方式影响基因组.虽然目前基因组中仍然有少数L1保留转座能力,大部分L1由于积累了大量的突变而失去了转座能力.正常组织中L1究竟呈现怎样的表达状况,是否发挥不依赖转座作用的功能,目前尚缺乏最基本的了解.通过采用高通量测序方法,我们在转录组水平研究了大鼠不同发育期大脑皮质中一类不具备转座活性的全长非完整L1(FLnI-L1)的表达模式,分析了FLnI-L1的表达与邻近基因的关系.同时,通过全基因组水平分析与FLnI-L1表达趋势一致的基因,我们推测FLnI-L1可能参与调控与神经发育有关的信号通路.本研究揭示了大鼠大脑皮质发育过程中FLnI-L1的表达模式,为进一步研究L1的生物学功能奠定了基础.

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