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科学通报  2011 

竞争双标记时间分辨荧光免疫分析法同时测定猪肉组织中氯霉素和莱克多巴胺研究

, PP. 304-308

Keywords: 竞争双标记时间分辨荧光免疫分析,氯霉素,莱克多巴胺,残留,猪肉组织

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Abstract:

基于竞争双标记时间分辨荧光免疫分析技术,建立了一种新的同时测定猪肉组织中氯霉素(CAP)和莱克多巴胺(RAC)的方法.方法对CAP检出限和加标回收率分别为0.06ng/g和102%~121%(加标水平为0.1~5ng/gCAP),对RAC检出限和加标回收率分别为0.25ng/g和69.8%~85.8%(加标水平为1~10ng/gRAC).对18个猪肉样品的分析表明,本研究所建立的竞争双标记时间分辨荧光免疫分析法的测定结果与ELISA及GC-MS法一致,相关系数在0.92~0.98之间.

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