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科学通报  2011 

青蒿琥酯对人白血病K562细胞凋亡抑制蛋白表达的影响

DOI: 10.1360/972010-2003, PP. 828-831

Keywords: 青蒿琥酯,IAP,家族,凋亡,caspase-3,PARP,cleavage

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Abstract:

用Westernblot法检测青蒿琥酯对白血病K562细胞的X染色体连锁凋亡抑制因子(X-linkedinhibitorofapoptosisprotein,XIAP)、存活素(Survivin)、细胞凋亡抑制蛋白1(cellularIAP1,cIAP-1)和细胞凋亡抑制蛋白2(cellularIAP2,cIAP-2)表达的影响,以及对K562细胞半胱氨酸天冬氨酸酶3(caspase-3)和多聚ADP-核糖聚合酶裂解片段(polyADP-ribosepolymerasecleavage,PARPcleavage)的作用.青蒿琥酯能够下调XIAP,Survivin和cIAP-2的表达,上调caspase-3活化亚基(17kD)和PARPcleavage(85kD)的表达.青蒿琥酯通过下调XIAP,Survivin和cIAP-2的表达来诱导白血病细胞凋亡,caspase-3也与诱导白血病细胞凋亡有关.

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