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科学通报  2011 

电场调控载药丝素蛋白微球的制备

DOI: 10.1360/972010-1953, PP. 1013-1018

Keywords: 丝素蛋白,电场,微球,药物缓释,生物相容性

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Abstract:

通过控制丝素蛋白在溶液中的构象,在低于1wt%的浓度条件下,获得由纳米或微米球组成的丝素蛋白电凝胶,并通过调节制备参数,初步实现了丝素蛋白微球在200nm~3μm之间的尺寸调控.以异硫氰酸荧光素标记的牛血清蛋白(FITC-BSA)作为模型药物,载药实验结果表明,在电场作用下,随着丝素蛋白在正极自组装形成微球,带有负电荷的药物在正极富集,并包覆进丝素蛋白微球,初始载药率可达75%以上,药物能在120h内缓慢均衡释放.考虑到温和的制备条件(低压电场、室温或者低温、水环境)、丝素蛋白的可降解性及其同药物的良好相容性,电场调控制备丝素蛋白微球体系有望成为缓释带负电荷的蛋白质和基因药物的优良载体.

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