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科学通报  2011 

一个编码含VQ模序蛋白的基因AtARVQ1参与拟南芥对砷酸盐的响应调控

DOI: 10.1360/972011-494, PP. 1891-1898

Keywords: 拟南芥,砷酸盐,VQ,模序,超表达,砷抗性

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Abstract:

砷酸盐(AsⅤ)是一种对包括人类和植物在内大部分生物具有剧毒的重金属.结果显示,编码含植物特有VQ模序蛋白基因AtARVQ1(arsenate-repressedVQmotif-containingprotein1)参与拟南芥对AsⅤ应答和抗性调控.结果表明,砷酸盐胁迫强烈抑制AtARVQ1基因在拟南芥中的转录表达.进一步利用组成型启动子CaMV35S驱动AtARVQ1基因在拟南芥中的表达,获得在砷酸盐处理条件下增强AtARVQ1基因转录的超表达植株,发现超表达AtARVQ1可以明显提高拟南芥对砷酸盐的抗性水平.系统进化分析发现,含VQ模序结构的AtARVQ1同源基因为植物特有,并进化出两大分支4个子分支,这类同源基因在双子叶植物和苔藓中分布于两大分支上,而在单子叶植物中仅分布在同一子分支上.这些结果表明,AtARVQ1基因在拟南芥对砷酸盐的抗性应答上有着重要作用,而其同源基因也可能在其他植物对砷胁迫应答中发挥作用.

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