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科学通报  2011 

基于微流控芯片构建一种新的体外血管生成模型

, PP. 2319-2327

Keywords: 血管生成,微流控系统,微环境,促血管生长因子

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Abstract:

血管生成在许多生理和病理过程中发挥着重要作用,但血管生成的机理仍不清楚.因此,为探明血管生成机理及开发"血管生成相关"疾病的治疗方法,在体外构建一个合适的血管生成模型是十分必要的.基于微流控系统构建了一种新型体外血管生成模型,该系统不仅能为内皮细胞生长提供一种近似于在体的微环境,并能实时监测内皮细胞对其微环境所发生变化的响应.为评价该系统用于血管生成模型建立的可行性和优越性,考察了促血管生长因子对内皮细胞增殖、迁移和管样结构形成能力的影响.研究结果表明,在促血管生长因子的诱导作用下,内皮细胞在三维基质材料中的增殖能力大大提高(提高了59.12%);在促血管生长因子浓度梯度的诱导作用下,内皮细胞定向从低浓度往高浓度侵入基质胶且形成管腔样结构.以上结果表明,该系统不仅能为血管生成机理的阐明提供一个良好的研究平台,还能为促血管生成药物或者抗血管生成药物的筛选提供一个合适的筛选平台.

References

[1]  1 Folkman J. Angiogenesis: An organizing principle for drug discovery? Nat Rev Drug Discov, 2007, 6: 273-286
[2]  2 Banfi A, von Degenfeld G, Blau H M. Critical role of microenvironmental factors in angiogenesis. Curr Atheroscler Rep, 2005, 7:227-234??
[3]  3 Semenza G L. Vasculogenesis, angiogenesis, and arteriogenesis: Mechanisms of blood vessel formation and remodeling. J Cell Biochem,2007, 102: 840-847??
[4]  4 柏金根, 杨健, 朱敦皖, 等. 生长因子及其体内微环境的多重相互作用. 科学通报, 2006, 51: 761-769
[5]  5 Folkman J. Angiogenesis. Annu Rev Med, 2006, 57: 1-18??
[6]  6 Carmeliet P. Angiogenesis in life, disease and medicine. Nature, 2005, 438: 932-936??
[7]  8 Jain R K, Schlenger K, Hockel M, et al. Quantitative angiogenesis assays: Progress and problems. Nat Med, 1997, 3: 1203-1208??
[8]  13 Shamloo A, Ma N, Poo M M, et al. Endothelial cell polarization and chemotaxis in a microfluidic device. Lab Chip, 2008, 8: 1292-1299??
[9]  14 Chung S, Sudo R, Vickerman V, et al. Microfluidic platforms for studies of angiogenesis, cell migration, and cell-cell interactions. AnnBiomed Eng, 2010, 38: 1164-1177
[10]  18 Keenan T M, Folch A. Biomolecular gradients in cell culture systems. Lab Chip, 2008, 8: 34-57??
[11]  7 Ucuzian A A, Greisler H P. In vitro models of angiogenesis. World J Surg, 2007, 31: 654-663??
[12]  9 Staton C A, Stribbling S M, Tazzyman S, et al. Current methods for assaying angiogenesis in vitro and in vivo. Int J Exp Pathol, 2004, 85:233-248??
[13]  10 Vickerman V, Blundo J, Chung S, et al. Design, fabrication and implementation of a novel multi-parameter control microfluidic platformfor three-dimensional cell culture and real-time imaging. Lab Chip, 2008, 8: 1468-1477??
[14]  11 Carrion B, Huang C P, Ghajar C M, et al. Recreating the perivascular niche ex vivo using a microfluidic approach. Biotechnol Bioeng,2010, 107: 1020-1028??
[15]  12 Chung S, Sudo R, Mack P J, et al. Cell migration into scaffolds under co-culture conditions in a microfluidic platform. Lab Chip, 2009, 9:269-275??
[16]  15 Lawley T J, Kubota Y. Induction of morphologic differentiation of endothelial cells in culture. J Invest Dermatol, 1989, 93 (2 Suppl):59-61??
[17]  16 Folkman J, Haudenschild C. Angiogenesis in vitro. Nature, 1980, 288: 551-556??
[18]  17 Lamalice L, Boeuf F L, Huot J. Endothelial cell migration during angiogenesis. Circ Res, 2007, 100: 782-794??

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