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科学通报  2012 

镧和钆对DU145细胞诱导的前成骨细胞和破骨细胞分化的影响

DOI: 10.1360/csb2012-57-6-418, PP. 418-423

Keywords: ,,前列腺癌细胞,成骨细胞,破骨细胞

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Abstract:

为探索稀土化合物在防治前列腺癌骨转移方面的潜在药理作用,研究了镧(La)和钆(Gd)对前列腺癌细胞DU145诱导的成、破骨细胞分化的影响.实验结果表明,DU145细胞的条件培养基能够促进小鼠前成骨细胞MC3T3-e1的增殖、ALP活性和钙沉积,而且经La处理后作用进一步加强.然而Gd处理后的DU145细胞条件培养基组对MC3T3-e1的ALP活性和钙沉积影响并不显著.RT-PCR实验表明,La可能通过上调DU145细胞分泌的细胞因子BMP6表达,抑制Noggin的表达进而促进成骨细胞的增殖和分化;而且成骨细胞的增殖、分化与JNK信号转导通路有关.此外,还观察到La能够抑制DU145细胞诱导的破骨细胞生成,并且通过下调前列腺癌细胞RANKL的表达而起到抑制作用.然而Gd对DU145细胞诱导的破骨细胞生成的影响并不显著.

References

[1]  1 Coleman R E. Clinical features of metastatic bone disease and risk of skeletal morbidity. Clin Cancer Res, 2006, 12: 6243s–6249s??
[2]  3 Guise T A, Mohammad K S, Clines G, et al. Basic mechanisms responsible for osteolytic and osteoblastic bone metastases. Clin Cancer Res, 2006, 12: 6213s–6216s??
[3]  4 Ibrahim T, Flamini E, Mercatali L, et al. Pathogenesis of osteoblastic bone metastases from prostate cancer. Cancer, 2010, 116: 1406– 1418??
[4]  5 Roato I, D’Amelio P, Gorassini E, et al. Osteoclasts are active in bone forming metastases of prostate cancer patients. PLoS ONE, 2008, 3: e3627??
[5]  7 Shailendra K. Lanthanum and its rapidly emerging role as an anti-carcinogenic agent. J Cell Biochem, 2009, 106: 193??
[6]  8 王芃, 黄健, 张天蓝, 等. 氯化镧抑制人转移型雄性激素非依赖性前列腺癌细胞DU145的生长和迁移. 科学通报, 2010, 55: 2289– 2295
[7]  9 Wang P, Zou X M, Huang J, et al. Gadolinium inhibits prostate cancer PC3 cell migration and suppresses osteoclast differentiation in vitro. Cell Biol Int, 2011, 35: 1159–1167??
[8]  10 Boyle W J, Simonet W S, Lacey D L. Osteoclast differentiation and activation. Nature, 2003, 423: 337–342??
[9]  13 Liu H, Liu Y, Viggeswarapu M, et al. Activation of c-Jun NH(2)-terminal kinase 1 increases cellular responsiveness to BMP-2 and decreases binding of inhibitory Smad6 to the type I BMP receptor. J Bone Miner Res, 2011, 26: 1122–1132??
[10]  14 Lacey D L, Timms E, Tan H L, et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell, 1998, 93: 165–176??
[11]  2 Casimiro S, Guise T A, Chirgwin J. The critical role of the bone microenvironment in cancer metastases. Mol Cell Endocrin, 2009, 310: 71–81??
[12]  6 Fricker S P. The therapeutic application of lanthanides. Chem Soc Rev, 2006, 35: 524–533??
[13]  11 Bentley H, Hamdy F C, Hart K A, et al. Expression of bone morphogenetic proteins in human prostatic adenocarcinoma and benign prostatic hyperplasia. Br J Cancer, 1992, 66: 1159–1163??
[14]  12 Hamdy F C, Autzen P, Robinson M C, et al. Immunolocalization and messenger RNA expression of bone morphogenetic protein-6 in human benign and malignant prostatic tissue. Cancer Res, 1997, 57: 4427–4431
[15]  15 Brown J M, Corey E, Lee Z D, et al. Osteoprotegerin and rank ligand expression in prostate cancer. Urology, 2001, 57: 611–616??

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