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科学通报  2012 

微生物油脂花生四烯酸产生菌离子束诱变和发酵调控

DOI: 10.1360/972011-2199, PP. 883-890

Keywords: 单细胞油脂,高山被孢霉菌,离子注入,花生四烯酸,生物柴油

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Abstract:

某些微生物是高质量油脂的生产者.微生物油脂不仅有益健康,而且是生物柴油潜在的油脂来源.中国是个油脂资源缺乏的国家,开发微生物源油脂具有重要的意义.本文以产油微生物——高山被孢霉菌为出发菌,以单细胞油脂多不饱和脂肪酸产率为筛选目标,采用二步离子束诱变-筛选的策略,获得了高产菌株.研究了高产菌株的营养需求,创建了重复利用提油后的残体(菌粕)合成微生物油脂的技术.针对丝状真菌高密度发酵的传能和传质问题,研制了6×50和4×200m3大容量专用反应器组,单位发酵容积生物量达38.2g/L(发酵液)、油脂20.67g/L.其中具有重要生理活性的花生四烯酸产率最高达9.89g/L,平均为8.97g/L.花生四烯酸提取和精炼后的残油转化为生物柴油,主要指标达到国家生物柴油标准.

References

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[2]  Ratledge C. Microbial production of c-linolenic acid. In: Akoh C.[J].ed. CRC Handbook of Functional Lipids. Boca Raton, FL: CRC Press.2005,:-
[3]  Metz J G, Roessler P, Facciotti D, et al. Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science, 2001, 293: 290-293
[4]  Carlson S E, Werkman S H, Peeples J M, et al. Arachidonic acid status correlates with first year growth in preterm infants. Proc Natl Acad Sci USA, 1993, 90: 1073-1077
[5]  Totani N, Someya K, Oba K. Industrial production of arachidonic acid by Mortierella. In: Kyle D J, Ratledge C, eds. Industrial Applications of Single Cell Oils. Champaign, IL: American Oil Chemists’ Society, 1992
[6]  余增亮. 离子束生物技术引论. 合肥: 安徽科学技术出版社.[J]..1998,:-
[7]  吴丽芳, 李红, 冯慧云, 等. 用低能氩离子束介导将水稻几丁质酶基因导入小麦. 科学通报, 2000, 21: 2316-2321
[8]  Dearnaley G. The SMMIB conferences: A retrospective. Surf Coat Technol, 2007, 201: 8004-8005
[9]  Feng H Y, Wu L J, Yu Z L, et al. Survival of mammalian cells under high vacuum condition for ion bombardment. Cryobiology, 2004, 49:241-249
[10]  Shu Q Y. A Summary of the International Symposium on Induced Mutations Plants. In: Induced Plant Mutations in the Genomics Era. Rome: Food and Agriculture Organizations Press, 2009
[11]  Feng H Y, Yu Z L, Chu P K. Ion implantation of organisms. Mater Sci Engin R, 2006, 54: 49-120
[12]  Yang J B, Wu L J, Li L, et al. Sequence analysis of lacz- mutation induced by ion beam in double-stranded M13mp18 DNA. Sci China Ser C-Life Sci, 1997, 40: 107-112
[13]  邵春林, 余增亮. 低能离子辐照的刺激效应模型. 核技术, 1996, 6: 321-325
[14]  Yuan C L, Wang J, Shang Y, et al. Production of arachidonic acid by Mortierella alpina I49-N18. Food Technol Biotechnol, 2002, 40: 311-315
[15]  王志明, 唐孝鹏, 张玉良, 等. 重复利用高山被孢霉菌粕制备花生四烯酸的方法. 中国专利, ZL 2009 1 0063369.6
[16]  凌关庭. 保健食品原料手册. 北京: 化学工业出版社, 2003
[17]  Ratledge C. Fatty acid biosynthesis in microorganisms being used for Single Cell Oil production. Biochimie, 2004, 86: 807-815
[18]  Cohen Z.[J].Ratledge C. Single Cell Oils. Champaign, IL: AOCS Press.2005,:-
[19]  Ratledge C, Wynn J P. The biochemistry and molecular biology of lipid accumulation in Oleaginous microorganisms. Adv Appl Microbiol,2002, 51: 1-51
[20]  Wynn J P, Hamid A A, Ratledge C. The role of malic enzyme in the regulation of lipids accumulation in filamentous fungi. Microbiology,1999, 145: 1911-1917
[21]  Yu Z, Deng J, He J, et al. Mutation breeding by ion implantation. Nucl Instrum Methods Phys Res B, 1991, 59/60: 705-708
[22]  Yu Z, Yang J, Wu Y, et al. Transferring Gus gene into intact rice cells by low energy ion beam[J].Nucl Instrum Methods Phys Res B.1993,80/81:1328-1331
[23]  杨剑波, 吴李君, 吴家道, 等. 应用低能离子束介导法获得水稻转基因植株. 科学通报, 1994, 16: 1530-1534
[24]  宋道军, 余汛. 离子束介导转基因提高E. coli UV 敏感菌株的DNA 损伤修复能力. 科学通报, 1999, 18: 1968-1972
[25]  Anuntalabhochai S, Chandej R, Phanchaisri B, et al. Ion-beam-induced deoxyribose nucleic acid transfer. Appl Phys Lett, 2001, 78:2393-2395
[26]  Oztarhan A. A message from the Conference Chairman. In: Proceeding of the 14th International Conference on Surface Modification of Materials by Ion Beams. Surf Coat Technol, 2007, 201: 8003
[27]  Xu A, Yao J, Yu L, et al. Mutation of Gluconobacter oxydans and Bacillus megatwrium in a two-step process of L-ascorbic acid manufacture by ion beam. J Appl Microbiol, 2004, 96: 1317-1323

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