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科学通报  2012 

显微拉曼光谱法定量检测碳氧血红蛋白饱和度

DOI: 10.1360/972011-2289, PP. 1222-1226

Keywords: 拉曼光谱,碳氧血红蛋白,氧合血红蛋白,一氧化碳,偏最小二乘法,间隔偏最小二乘法

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Abstract:

采用显微拉曼光谱技术对不同饱和度碳氧血红蛋白(HbCO)进行测定,通过偏最小二乘法(PLS)和间隔偏最小二乘法(IPLS)建立模型,得出线性回归方程.结果表明,经过筛选变量信息所建立的间隔偏最小二乘模型(IPLS),当血红蛋白系列样品中碳氧血红蛋白含量为0~100%时,线性相关系数的平方为0.99,检测限为5.37%,完全能满足临床和法医学上CO中毒程度的检测.此方法具有快速、简便、直接、对样品无损等优点,有望成为检测碳氧血红蛋白饱和度的新方法.

References

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[2]  姜宴, 叶永红, 张宜凡, 等. 血中碳氧血红蛋白饱和度测定影响因素的研究. 法医学杂志, 2003, 19: 88-91
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[4]  Brunner H, Sussner H. Resonance Raman scattering on haemoglobin. Biochim Biophys Acta, 1973, 310: 20-31
[5]  沈高山, 谷怀民, 闫天秀, 等. 亚硝酸钠和氧合血红蛋白反应的拉曼光谱. 中国激光, 2008, 35: 1432-1436
[6]  Yamamoto T, Palmer G. The valence and spin state of iron in oxyhemoglobin as inferred from resonance Raman spectroscopy. J Biol Chem, 1973, 248: 5211-5213
[7]  Rousseau D L, Ching Y C, Brunori M, et al. Axial coordination of ferric aplysia myoglobin. J Biol Chem, 1989, 264: 7878-7881
[8]  Shafer-Peltier K E, Haynes C L, Glucksberg M R, et al. Toward a glucose biosensor based on surface-enhanced Raman scattering. J Am Chem Soc, 2003, 125: 588-593
[9]  Norgaard L, Saudland A, Wagner J, et al. Interval partial least-squares regression (iPLS): A comparative chemometric study with an example from near-infrared spectroscopy. Appl Spectrosc, 2000, 54: 413-419
[10]  司法鉴定技术规范. 血中碳氧血红蛋白饱和度的测定分光光度法. SF/ZJD0107010-2011
[11]  张介克, 沈敏, 刘伟. 一氧化碳血红蛋白含量的定量检测及估价. 法医学杂志, 1986, 2: 27-30
[12]  杨娅, 屠一锋. 可见分光光度法快速检测碳氧血红蛋白饱和度. 现代科学仪器, 2008, 34: 24-25
[13]  谭家镒, 杨鸣. 四阶导数光谱法测定血液碳氧血红蛋白饱和度. 中国法医学杂志, 1988, 3: 206-209
[14]  贺浪冲. 法医毒物分析. 北京: 人民卫生出版社.[J]..2004,:-
[15]  乔静. 毒品和毒物检验. 北京: 中国人民公安大学出版社, 2003. 697-701
[16]  刘颂豪, 孟耀勇. DNA 分子的远红外和低频喇曼光谱的研究. 红外与毫米波学报, 1991, 10: 487-492
[17]  廖昱博, 孟耀勇, 雷浩东, 等. PolyA, PolyU 及双链复合物PolyA·PolyU 的拉曼光谱研究. 光谱学与光谱分析, 2007, 27: 940-943
[18]  Ewen S.[J].Geoffrey D J. Modern Raman Spectroscopy-A Practical Approach. Chichester: John Wiley & Sons Ltd.2005,:-
[19]  Virkler K, Lednev I K. Raman spectroscopic signature of blood and its potential application to forensic body fluid identification. Anal Bioanal Chem, 2010, 396: 525-534
[20]  Bergholt M S, Hassing S. Quantification of C-reactive protein in human blood plasma using near-infrared Raman spectroscopy. Analyst,2009, 134: 2123-2127

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