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科学通报  2012 

检测巯基物质的光学探针研究进展

DOI: 10.1360/972012-433, PP. 1462-1471

Keywords: 巯基物质检测,谷胱甘肽,半胱氨酸,高半胱氨酸,光学探针

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Abstract:

巯基物质是生物体内主要的抗氧化剂,并以多种形式广泛存在.定量检测巯基物质在生化研究和相关疾病诊断方面意义突出.目前,利用高选择性、高灵敏度的光学探针开展巯基物质的检测研究已成为前沿课题之一.其中,荧光探针由于能够实现活体的原位、实时成像尤其受到关注.本文基于光学探针与巯基物质(主要包括谷胱甘肽、半胱氨酸和高半胱氨酸)的不同反应机理,就近年来该领域的研究新进展做了较系统的评述.所涉及的反应机理主要有双键的麦克尔加成、苯环的亲核取代、金属的配位络合、巯基物质参与的氧化反应和成环反应、以及静电作用等.此外,还讨论了检测巯基物质的光学探针存在的问题,并展望了其发展趋势与应用前景.

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