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科学通报  2012 

基于原子力显微镜微切削加工技术的癌细胞内部弹性模量计算

, PP. 3533-3537

Keywords: 原子力显微镜,微切削加工,内部弹性模量

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Abstract:

癌细胞机械力学特性的测量为癌症的诊断、预防、治疗、病变机理等方面的研究提供全新的手段和巨大的潜在空间.本文建立细胞和针尖之间的接触压力和针尖在细胞上切入深度的计算模型,利用原子力显微镜(AFM)单晶硅针尖对固定的卵巢癌细胞(UACC1598)和结肠癌细胞(NCI-H716)进行微切削逐层去除.结果表明由于不同细胞的结构和机械性质的差异,相同的加载力对不同的细胞材料进行切削加工时,细胞和针尖之间的接触压力和针尖在细胞上的切入深度差异较大.细胞表面微切削去除范围为8μm×8μm,在细胞上逐层去除材料时的加载力从17.523到32.126μN逐渐增大,去除后对每层不同位置进行弹性模量测量,得到细胞内部弹性模量分布,细胞内部弹性模量与细胞表面弹性模量差分别为卵巢癌细胞0.288±0.08kPa,结肠癌细胞0.376±0.16kPa.用这种微切削去除方法可以测量细胞内部细胞骨架细胞器等各结构的力学特性,为疾病的诊断和治疗提供更精确的实验数据.

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