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科学通报  2015 

EXO70在拟南芥和水稻基因组中的倍增

DOI: 10.1360/N972014-00853, PP. 38-51

Keywords: EXO70,pfam03081,基因倍增,密码子偏好性

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Abstract:

作为胞泌复合体的一个重要组件,EXO70在人类、小鼠及酵母中均由单基因编码.为研究EXO70在拟南芥和水稻基因组中倍增的异同,从拟南芥和水稻基因组中钓取所有的EXO70家族基因,分析后发现,拟南芥中包含23个EXO70基因,其中15个基因位于染色体的负链;选择性剪接的存在共产生27种成熟的mRNA.水稻中共发现47个EXO70基因座,它们分别分散于12条染色体的正负链,其中的29个基因位于正链;分别包含OsEXO70H3和OsEXO70H4在内的11号染色体和12染色体的5′端存在一段约2×106bp区段的序列高度同源;OsEXO70基因倍增的复杂性高于拟南芥EXO70.EXO70基因在拟南芥和水稻中均存在密码子使用偏好性,AtEXO70和OsEXO70的密码子偏好性存在较大的差异.Pfam03081是EXO70蛋白质共有的结构域,且在三维结构上极为保守,这可能决定不同的EXO70蛋白存在相同或相近的功能.结果表明,EXO70在拟南芥和水稻2个物种进化中均存在多次加倍,OsEXO70倍增的次数、方式和复杂性都高于AtEXO70;高度保守的Pfam03081可能是决定EXO70蛋白功能的关键因素;像其他基因一样,EXO70同义密码子的偏性模式存在明显的物种特异性.

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