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科学通报  2013 

拟南芥中ABR蛋白家族在ABA和逆境胁迫信号转导过程中功能分析

, PP. 2751-2761

Keywords: ABA反应蛋白,GRAM结构域,ABA,萌发,拟南芥

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Abstract:

植物激素脱落酸(abscisicacid,ABA)在调节植物生长、发育,以及植物在应对生物与非生物胁迫的反应等各方面都发挥着非常关键的作用.已知ABA调控具有ABRE(ABAresponsiveelement)顺式作用元件的基因表达和相应的生理反应.生物信息学分析表明,拟南芥ABA反应蛋白(ABA-responsiveprotein,ABR)家族含有GRAM结构域,而且这些基因上游均具有ABRE应答元件.为了分析这些基因可能的功能,本研究鉴定了ABR家族的T-DNA插入纯合突变体,并构建了abr1,abr2,abr3双突变和三突变体.abr1,abr2,abr3单突变体没有表现出任何生长及其他生理表型,而abr1,abr2,abr3双突变和三突变体在种子萌发方面对ABA、氯化钠、甘露醇及葡萄糖均显示出不敏感的表型.ABR1蛋白在胞质内呈点状分布,可能定位于内膜系统,而ABR2和ABR3蛋白均定位于细胞质中.另外,ABR1,ABR2和ABR3基因在植物多种组织中均有表达,且受包括ABA在内的多种非生物胁迫诱导,而它们的表达在aba2(abscisicacid(ABA)-deficientmutant2,ABA缺失突变体2),abi1(abscisicacidinsensitive1,ABA不敏感突变体1),abi2(abscisicacidinsensitive2,ABA不敏感突变体2)缺失突变体中明显下调.这些结果暗示了ABR家族蛋白参与ABA信号转导过程,且位于abi1,abi2的下游.

References

[1]  1 Cutler S R, Rodriguez P L, Finkelstein R R, et al. Abscisic acid: Emergence of a core signaling network. Annu Rev Plant Biol, 2010, 61: 651-679
[2]  2 Gosti F, Beaudoin N, Serizet C, et al. ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell, 1999, 11: 1897-1910
[3]  3 Fujii H, Chinnusamy V, Rodrigues A, et al. In vitro reconstitution of an abscisic acid signalling pathway. Nature, 2009, 462: 660-664
[4]  4 Ma Y, Szostkiewicz I, Korte A, et al. Regulators of PP2C phosphatase activityfunction as abscisic acid sensors. Science, 2009, 324: 1064-1068
[5]  6 He Y, Gan S. A novel zinc-finger protein with a proline-rich domain mediates ABA-regulated seed dormancy in Arabidopsis. Plant Mol Biol, 2004, 54: 1-9
[6]  7 Giraudat J, Hauge B M, Valon C, et al. Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell, 1992, 4: 1251-1261
[7]  8 Van Z M, Carles A, Li Y, et al. Control and consequences of chromatin compaction during seed maturation in Arabidopsis thaliana. Plant Signal Behav, 2012, 7: 338-341
[8]  10 Finkelstein R, Gampala S, Rock C. Abscisic acid signaling in seeds and seedlings. Plant Cell, 2002, 14 (Suppl): S15-S45
[9]  11 Schmitz N, Abrams S R, Kermode A R. Changes in ABA turnover and sensitivity that accompany dormancy termination of yellow cedar. J Exp Bot, 2002, 53: 89-91
[10]  15 Doerks T, Strauss M, Brendel M, et al. GRAM, a novel domain in glucosyltransferases, myotubularins and other putative membrane- associated proteins. Trends Biochem Sci, 2000, 25: 483-485
[11]  16 Berger P, Schaffitzel C, Berger I, et al. Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology- GRAM domain and a coiled-coil dimerization module. Proc Natl Acad Sci USA, 2003, 100: 12177-12182
[12]  17 Choudhury P, Srivastava S, Li Z, et al. Specificity of the myotubularin family of phosphatidylinositol-3-phosphatase is determined by the PH/GRAM domain. J Biol Chem, 2006, 281: 31762-31769
[13]  23 Kaplan B, Davydov O, Knight H, et al. Rapid transcriptome changes induced by cytosolic transients reveal ABRE-related sequences as Ca2+-responsive cis elements in Arabidopsis. Plant Cell, 2006, 18: 2733-2748
[14]  29 Luo M, Wang Y Y, Liu X, et al. HD2C interacts with HDA6 and is involved in ABA and salt stress response in Arabidopsis. J Exp Bot, 2012, 63: 3297-3306
[15]  33 Jiang S Y, Cai M, Ramachandran S. The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domain-containing protein. Plant Mol Biol, 2005, 57: 835-853
[16]  37 Elena C M, Mar C, Crisanto G. GEM, a novel factor in the coordination of cell division to cell fate decisions in the Arabidopsis epidermis. Plant Signal Behav, 2007, 2: 494-495
[17]  38 Takemiya A, Yamauchi S, Yano T, et al. Identification of a regulatory subunit of protein phosphatase 1 which mediates blue light signaling for stomatal opening. Plant Cell Physiol, 2012, 54: 25-35
[18]  40 Lin P C, Hwang S G, Endo A, et al. Ectopic expression of ABSCISIC ACID 2/GLUCOSE INSENSITIVE 1 in Arabidopsis promotes seed dormancy and stress tolerance. Plant Physiol, 2007, 143: 745-758
[19]  42 Stefan H, Michele M, Juan P S, et al. Genome-wide gene expression profiling in Arabidopsis thaliana reveals new targets of abscisic acid and largely impaired gene regulation in the abi1-1 mutant. J Cell Sci, 2002, 115: 4891-4900
[20]  5 Park S Y, Hiroaki F, Yang Z, et al. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science, 2009, 324: 1068-1071
[21]  9 Park J, Lee N, Kim W, et al. ABI3 and PIL5 collaboratively activate the expression of SOMNUS by directly binding to its promoter in imbibed Arabidopsis seeds. Plant Cell, 2011, 23: 1404-1415
[22]  12 Xiong L, Gong Z, Rock C D. Modulation of abscisic acid signal transduction and biosynthesis by an Sm-like protein in Arabidopsis. Dev Cell, 2001, 1: 771-781
[23]  13 Liu J H, Luo M, Cheng K J, et al. Identification and characterization of a novel barley gene that is ABA-inducible and expressed specifically in embryo and aleurone. J Exp Bot, 1999, 50: 727-728
[24]  14 Banno H, Chua N H. Characterization of the Arabidopsis formin-like protein AFH1 and its interacting protein. Plant Cell Physiol, 2000, 41: 617-626
[25]  18 Tsujita K, Itoh T, Ijuin T, et al. Myotubularin regulates the function of the late endosome through the GRAM domain-phosphatidylinositol 3,5-bisphosphate interaction. J Biol Chem, 2004, 279: 13817-13824
[26]  19 Masahide O, Dirk W, Takeshi N, et al. Peroxisome degradation requires catalytically active sterol glucosyltransferase with a GRAM domain. EMBO J, 2003, 22: 3231-3141
[27]  20 Shu Y J, Rengasamy R, Srinivasan R. Comparative transcriptional profiling and evolutionary analysis of the GRAM domain family in eukaryotes. Dev Biol, 2008, 314: 418-432
[28]  21 Yoo S D, Cho Y H, Sheen J. Arabaidopsis mesophyll protoplasts: A versatile cell system for transient gene expression analysis. Nat Protoc, 2007, 2: 1565-1572
[29]  22 Nakashima K, Fujita Y, Katsura K, et al. Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis. Plant Mol Biol, 2006, 60: 51-68
[30]  24 Choi H, Hong J, Ha J, et al. ABFs, a family of ABA-responsive element binding factors. J Biol Chem, 2000, 275: 1723-1730
[31]  25 Kang J Y, Choi H I, Im M Y, et al. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell, 2002, 14: 343-357
[32]  26 Oh S J, Song S I, Kim Y S, et al. Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol, 2005, 138: 341-351
[33]  27 Choi H I, Park H J, Park J H, et al. Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity. Plant Physiol, 2005, 139: 1750-1761
[34]  28 Yamaguchi S K, Shinozaki K. Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis. Mol Gen Genet, 1995, 247: 391-398
[35]  30 Gao G, Zhang S, Wang C, et al. Arabidopsis CPR5 independently regulates seed germination and postgermination arrest of development through LOX pathway and ABA signaling. PLoS One, 2011, 6: e19406
[36]  31 Du S C, Byung K H. Proteomics and functional analyses of pepper abscisic acid-responsive 1 (ABR1), which is involved in cell death and defense signaling. Plant Cell, 2011, 23: 823-842
[37]  32 Rowland O, Ludwig A A, Merrick C J, et al. Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato. Plant Cell, 2005, 17: 295-310
[38]  34 Severine L, Bai Q L, Marie C A, et al. Vascular associated death 1, a novel GRAM domain-containing protein, is a regulator of cell death and defense responses in vascular tissues. Plant Cell, 2004, 16: 2217-2232
[39]  35 Somya D, Radomira V, Vaclav M, et al. Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants. Plant Growth Regul, 2010, 61: 231-242
[40]  36 Caro E, Castellano M M, Gutierrez C. A chromatin link that couples cell division to root epidermis patterning in Arabidopsis. Nature, 2007, 447: 213-217
[41]  39 Gonzalez G M, Apostolova N, Belles J M, et al. The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde. Plant Cell, 2002, 14: 1833-1846
[42]  41 Leung J, Merlot S, Giraudat J. The Arabidopsis abscisic acid-insensitive 2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell, 1997, 9: 759-771
[43]  43 Hai L S, Xiao J W, Wei H D, et al. Identification of an important site for function of the type 2C protein phosphatase ABI2 in abscisic acid signalling in Arabidopsis. J Exp Bot, 2011, 62: 5713-5725
[44]  44 Wan H C, Akira E, Li Z, et al. A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell, 2002, 14: 2723-2743

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