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科学通报  2013 

高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)分析水产品中游离态三价铝(Al3+)

, PP. 3812-3817

Keywords: 固相萃取,水产品,Al3+,HPLC-ICP-MS

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Abstract:

研究建立了固相萃取-高效液相色谱-电感耦合等离子体质谱联用技术(SPE-HPLC-ICP-MS)测定水产品中游离态三价铝(Al3+)的提取、净化及检测方法.选用CG5A阳离子保护柱和CS5A阳离子分析柱对样品中Al3+进行分离,流动相组成为0.040mol/LLiOH,0.0060mol/L吡啶-2.6-二羧酸(PDCA)和0.090mol/LCH3COOH(pH4.7);用流动相做提取剂,将样品置70℃恒温水浴振荡器浸提2h,浸提液经离心后,上清液用OasisMCX(3mL/60mg,30μm)固相萃取柱净化,固相萃取柱使用前用2mL甲醇和4mL去离子水活化和平衡,2mL0.075mol/L的HCL溶液洗脱目标组分.该提取净化方法能有效排除水产品中有机质的干扰,提高方法灵敏度.新建方法的回收率>89%,相对标准偏差3+的检测结果与化学试剂浸提法的分析结果一致.

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