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科学通报  2012 

基于金纳米颗粒等离子体共振吸收的典型四环素类药物分析方法

DOI: 10.1360/972011-834, PP. 52-58

Keywords: 金纳米颗粒,等离子体共振吸收,四环素类药物

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Abstract:

研究发现,在pH9.37的BR缓冲体系中,典型四环素类药物盐酸土霉素、盐酸多西环素、盐酸四环素、盐酸金霉素与氯金酸发生氧化还原反应生成的金纳米颗粒在500~550nm波长范围内显示出特征的等离子体共振吸收,且吸光度值与四环素类药物的浓度在一定范围内呈良好的线性关系,据此建立了测定四环素类药物的等离子体共振吸收分析新方法.该方法能分别检测0.5~20,0.5~16,0.5~16,0.5~20μmol/L范围的盐酸土霉素、盐酸多西环素、盐酸四环素和盐酸金霉素,对应的检出限分别为0.20,0.16,0.15,0.18μmol/L.对浓度为20.0μmol/L的盐酸土霉素进行了15次平行测定,其相对标准偏差为1.53%.该方法成功用于合成样和片剂中土霉素的测定,且根据溶液颜色变化实现了上述四环素类药物的三原色可视化定量检测.

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