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基于场-路复合模型的细胞内外膜跨膜电位时频特性

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Keywords: 场-路复合模型,细胞内外膜,脉冲电场,跨膜电位,时域分析,频域分析

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Abstract:

为研究脉冲电场的生物医学效应机理,基于多层介质电场模型建立了球形单细胞的场-路复合模型,提出了内外膜跨膜电位与外加电场之间传递函数的计算方法,分别分析了脉冲电场作用下内外膜跨膜电位的时域特性与频率特性。时域分析表明,不同的脉宽导致外加电场对内外膜具有不同的选择性作用。频域分析表明,细胞内外膜分别具有带通和低通滤波性能,在不同脉宽的脉冲电场(具有不同的频谱分布)作用下,将诱导出不同的生物医学效应。理论分析与医学实验结果均表明,场-路复合模型与电场模型和电路模型均吻合得很好,对电穿孔、凋亡等生物医学效应的诱导机理给出了初步统一的解释,为脉冲电场用于肿瘤治疗的机理研究和参数的合理选择提供了理论依据。

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