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菌物学报  2015 

根癌农杆菌介导的地衣型真菌Cladoniametacorallifera的转化

DOI: DOI:10.13346/j.mycosystema.140015, PP. 246-251

Keywords: Cladonia,metacorallifera,农杆菌介导的转化,增强型绿色荧光蛋白,潮霉素抗性

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Abstract:

以潮霉素抗性和增强型绿色荧光蛋白(enhancedgreenfluorescentprotein,EGFP)作为筛选标记,利用地衣型真菌Cladoniametacorallifera的菌丝,成功实现了根癌农杆菌介导的遗传转化,PCR检测证明转化子中存在潮霉素抗性基因,共聚焦显微镜检测到转化子菌丝能够产生绿色荧光,证明EGFP能够在trpC启动子控制下在地衣型真菌中表达。

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