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菌物学报  2015 

白色麝香霉挥发性有机化合物的抗菌活性和组成分析

DOI: DOI:10.13346/j.mycosystema.130274, PP. 269-277

Keywords: 麝香霉,系统发育,最适温度,气体成分,抗菌活性

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Abstract:

采用核糖体RNA转录间隔区(internaltranscribedspacerofribosomalRNA,ITSrRNA)、RNA聚合酶II(RNApolymeraseII,rpb2)和β-维管蛋白(beta-tubulin,tub1)基因系统发育分析法,鉴定了一株分离自浙江庆元的麝香霉ZJQY709,鉴定结果表明该菌为白色麝香霉Muscodoralbus。生长速率法测定结果显示该菌株最适生长温度为22–28℃。二分格培养皿对峙培养法测定该菌挥发性有机化合物的抑菌活性,结果表明其挥发性有机化合物对灰葡萄孢菌和立枯丝核菌的生长抑制作用较强,而对尖孢镰刀菌和终极腐霉的抑制作用较弱。固相微萃取/气质联用法分析该菌产生的挥发性有机化合物,结果显示其挥发性有机化合物成分复杂,主要气体成分为2-甲基丙酸、乙酸-2-甲基丙酯和1-丁醇-2-甲基乙酸。

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