全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
菌物学报  2015 

常见瓜类植物的内生真菌多样性

DOI: DOI:10.13346/j.mycosystema.140030, PP. 196-203

Keywords: 葫芦科植物,内生真菌,分离,相对多度,优势属

Full-Text   Cite this paper   Add to My Lib

Abstract:

为认识和掌握常见瓜类植物内生真菌多样性及其变化规律,对从4个地点采集的9种70株健康瓜类植物进行了内生真菌的分离,共获得534个菌株,总体分离率为20.9%。结合形态学和ITS序列分析,鉴定了分属于55个属的455个菌株,有8个属的真菌属于优势类群,相对多度最高的3个属依次为Colletotrichum、Fusarium和Rhizopycnis。Colletotrichum主要存在于植株地上部,Fusarium主要存在于根部和下部茎,Rhizopycnis主要存在于根部,表现出明显的部位差异。河南原阳不同瓜类植物样品中主要内生真菌类群的相对多度和内生真菌的多样性存在明显差异,蛇瓜中Colletotrichum的相对多度达44.7%,显著高于丝瓜中Colletotrichum的相对多度8.0%;黄瓜中Fusarium的相对多度达32.4%,远高于在其他瓜类植物中的相对多度,而在南瓜中Fusarium的相对多度最低,仅为3.0%。江苏无锡和河南原阳的丝瓜植株样品中内生真菌的类群组成有很大不同,表明植株生长的地理环境可能对内生真菌的组成和多样性具有显著影响。

References

[1]  Abeysinghe S, 2009. Use of nonpathogenic Fusarium oxysporum and rhizobacteria for suppression of Fusarium root and stem rot of Cucumis sativus caused by Fusarium oxysporum f. sp. radicis-cucumerinum. Archives of Phytopathology and Plant Protection, 42: 73-82 [本文引用:1]
[2]  Arnold AE, Henk DA, Eells RL, Lutzoni F, Vilgaly R, 2007. Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR. Mycologia, 99: 185-206 [本文引用:1]
[3]  Arnold AE, Maynard Z, Gilbert G, Coley PD, Kursar TA, 2000. Are tropical fungal endophytes hyperdiverse?Ecology Letters, 3: 267-274 [本文引用:1]
[4]  Camargo JA, 1992. Can dominance influence stability in competitive interactions?Oikos, 64: 605-609 [本文引用:1]
[5]  Carroll G, 1988. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology, 69: 2-29 [本文引用:1]
[6]  Carroll GC, Carroll FE, 1978. Studies on the incidence of coniferous needle endophytes in the Pacific Northwest. Canadian Journal of Botany, 56: 3034-3043 [本文引用:1]
[7]  Death RG, 1996. The effect of patch disturbance on stream invertebrate community structure: the influence of disturbance history. Oecologia, 108: 567-576 [本文引用:1]
[8]  Eschen R, Hunt S, Mykura C, Gange AC, Sutton BC, 2010. The foliar endophytic fungal community composition in Cirsium arvense is affected by mycorrhizal colonization and soil nutrient content. Fungal Biology, 114: 991-998 [本文引用:1]
[9]  Fang ZD, 1998. Research methods of plant pathology. China Agriculture Press, Beijing. 122-137(in Chinese) [本文引用:1]
[10]  Loro M, Valero-Jiménez CA, Nozawa S, Márquez LM, 2012. Diversity and composition of fungal endophytes in semiarid Northwest Venezuela. Journal of Arid Environments, 85: 46-55 [本文引用:1]
[11]  Mu LX, Niu YC, Deng H, 2010. The endophytic mycobiota in summer growing cucumber in Beijing. Mycosystema, 29: 214-221 (in Chinese) [本文引用:1]
[12]  Park CS, Paulitz TC, Baker R, 1988. Biocontrol of Fusarium wilt of cucumber resulting from interactions between Pseudomonas putida and nonpathogenic isolates of Fusarium oxysporum. Phytopathology, 78: 190-194 [本文引用:1]
[13]  Petrini O, 1991. Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS (eds. ) Microbial ecology of leaves. Springer-Verlag, New York. 179-197 [本文引用:1]
[14]  Schulz B, Guske S, Dammann U, Boyle C, 1998. Endophyte-host interactions. II. Defining symbiosis of the endophyte-host interaction. Symbiosis, 25: 213-227 [本文引用:1]
[15]  Schulz B, Wanke U, Draeger S, 1993. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycological Research, 97: 1447-1450 [本文引用:1]
[16]  Summerbell RC, 2005. Root endophyte and mycorrhizosphere fungi of black spruce Picea mariana, in a boreal forest habitat: in?uence of site factors on fungal distributions. Studies in Mycology, 53: 121-145 [本文引用:1]
[17]  Tian XL, Cao LX, Tan HM, Zeng QG, Jia YY, Han WQ, Zhou SN, 2004. Study on the communities of endophytic fungi and endophytic actinomycetes from rice and their antipathogenic activities in vitro. World Journal of Microbiology & Biotechnology, 20: 303-309 [本文引用:1]
[18]  Wang ZY, Wang M, Gao SF, Zhang M, Yuang HX, Li HL, 2008. Phyllosphere and endophytic microflora in greenhouse cucumber leaves. Chinese Journal of Ecology, 27: 425-428 (in Chinese) [本文引用:1]
[19]  Weising K, Nybom H, Wolff K, Meyer W, 1995. DNA fingerprinting in plants and fungi. CRC Press, Boca Raton, Florida. 1-322 [本文引用:1]
[20]  White TJ, Bruns T, Lee S, Taylor J, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds. ) PCR protocols: a guide to methods and applications. Academic Press, San Diego. 315-322 [本文引用:1]
[21]  Yan XN, Sikora RA, Zheng JW, 2011. Potential use of cucumber (Cucumis sativus L. ) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita. Journal of Zhejiang University Science B, 12: 219-225 [本文引用:1]
[22]  Yu SQ, Wang SZ, 1992. The vascular bundle fungiflora of watermelon. Acta Agriculturae Universitatis Henanensis, 26: 380-387 (in Chinese) [本文引用:1]
[23]  方中达(主编), 1998. 植病研究方法. 北京: 中国农业出版社. 122-137 [本文引用:1]
[24]  慕林轩, 牛永春, 邓晖, 2010. 北京夏植黄瓜内生真菌区系研究. 菌物学报, 29: 214-221 [本文引用:1]
[25]  王振跃, 汪敏, 高书锋, 张猛, 袁虹霞, 李洪连, 2008. 温室黄瓜叶部微生物区系. 生态学杂志, 27: 425-428 [本文引用:1]
[26]  于思勤, 王守正, 1992. 西瓜维管束真菌区系研究. 河南农业大学学报, 26: 380-387 [本文引用:1]

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133