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菌物学报  2015 

一色齿毛菌对百菌清的生物降解研究

DOI: DOI:10.13346/j.mycosystema.140044, PP. 473-481

Keywords: 卵菌杀菌剂,生物降解,白腐真菌,漆酶

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Abstract:

为了验证白腐真菌对杀菌剂——百菌清的降解能力,并初步探讨其降解机制,以平皿培养方式对70株白腐真菌菌株降解百菌清的能力进行验证和筛选,并选择降解作用突出的一色齿毛菌CerrenaunicolorY4941进行液体培养条件下对百菌清的降解。结果表明:卵菌杀菌剂没有对测试的白腐真菌产生抑制或杀灭作用,在所筛选的70株菌株中,一色齿毛菌CerrenaunicolorY4941、硬毛粗盖孔菌FunaliatrogiiW4289、桦褶孔菌LenzitesbetulinaY4962、藏红硬孔菌RigidoporuscrocatusD10146、裂褶菌SchizophyllumcommuneD9466和云芝栓孔菌TrametesversicolorW4614等菌株都对百菌清显示出较强的降解能力;对一色齿毛菌CerrenaunicolorY4941在液体培养条件下降解百菌清研究的实验结果表明,活菌(livingculture,LC)降解率>粗酶液(crudeenzymeliquor,CEL)降解率>灭活菌丝体(devitalizedmycelia,DM)降解率,经过活菌降解3d的样品中百菌清的残留率仅为1.92%;对培养液中漆酶活性测定结果显示,漆酶活性最高可达2195.55U/L。上述证明,杀菌剂百菌清不对白腐真菌产生抑制或杀灭作用,相反,一色齿毛菌CerrenaunicolorY4941等菌株对其有很强的降解能力,在杀菌剂生物降解领域有潜在的应用前景。

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