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菌物学报  2015 

基于rDNAITS序列对绒泡菌目黏菌系统发育的探讨

DOI: DOI:10.13346/j.mycosystema.140054, PP. 424-433

Keywords: 黏菌,核糖体DNA内部转录间隔区,分子进化,最大似然法,贝叶斯分析

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Abstract:

绒泡菌目Physarida是黏菌纲Myxogastria最大的一个目,对其系统发育关系的研究一直是根据形态特征。为了从分子水平探讨绒泡菌目乃至黏菌纲的系统发育关系,以黏菌rDNAITS通用引物对绒泡菌目5属8种黏菌的rDNAITS进行扩增和测序,结合GenBank中已有的黏菌rDNAITS序列,利用贝叶斯推断法(Bayesianinference,BI)和最大似然法(Maximumlikelihood,ML)构建系统发育树。结果表明:绒泡菌目不同物种的rDNAITS区在碱基组成和长度上差异明显,长度为777–1445bp,G+Cmol%在53.4%–61.9%之间。绒泡菌目与发网菌目Stemonitida聚类为两个明显的分支,在绒泡菌目分支上,绒泡菌科Physaraceae和钙皮菌科Didymiaceae各聚为一支,支持了形态学上以孢丝是否具有石灰质为依据区分这两个科的观点。由多份不同地理来源的鳞钙皮菌Didymiumsquamulosum材料组成的钙皮菌科又形成3个分支,证实了这个形态种是由地域来源广泛、繁殖亲和性各异和遗传变异较大的不同生物种组成的复合体。

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