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菌物学报  2015 

新疆沙冬青AM真菌群落结构与遗传多样性分析

DOI: DOI:10.13346/j.mycosystema.140050, PP. 375-385

Keywords: AM真菌丰度,菌群结构,遗传多样性,新疆沙冬青

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Abstract:

以新疆乌恰县4个样地新疆沙冬青Ammopiptanthusnanus根围3个土层土壤为研究对象,利用Nested-PCR-DGGE技术,结合DGGE图谱分析、DNA克隆测序及系统发育分析,研究新疆沙冬青AM真菌菌群结构与遗传多样性特征,为新疆沙冬青根围功能菌群研究奠定基础。结果表明,不同样地和土层,AM真菌具有不同DGGE指纹图谱特征,AM真菌丰度、优势度及其多样性指数均有差异,其中上阿图什30–40cm土层AM真菌丰度和多样性指数最高,分别为20和3.48。DGGE条带测序和系统发育分析显示,全部序列归为2属14种,球囊霉属Glomus是其优势属,其中UnculturedGlomus(AB698612)和UnculturedGlomus(AB698616)普遍存在于4样地3个土层,Glomussp.(FN429104)和UnculturedGlomus(KC797120)是上阿图什30–40cm土层特有种,Glomussp.(EU332717),Rhizophagusintraradices(FR750206)和UnculturedGlomus(AB698616)是新疆沙冬青根围优势种。G.indicum和Rh.intraradices在形态学鉴定中未出现,说明分子生物学技术对真菌多样性研究起着补充和完善的作用。

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