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菌物学报  2015 

三七根腐病株根际土壤真菌群落组成与碳源利用特征研究

DOI: 10.13346/j.mycosystema.130164, PP. 65-74

Keywords: 三七,根腐病,真菌群落,末端限制性酶切片段长度多态性分析(T-RFLP),Biolog技术

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Abstract:

根腐病是药用植物三七Panaxnotoginseng的多发性病害,而土壤真菌群落组成与根腐病的发生存在一定的联系。利用末端限制性酶切片段长度多态性分析和Biolog技术研究了云南文山三七种植园健康和发病三七植株根际土壤真菌群落结构和碳源利用特征,结果表明健康和发病植株的根际土壤真菌多样性和代谢活性差异不显著,但是真菌群落组成存在差异。健康和发病植株根际土壤都以小茎点霉Phomaexigua及镰孢菌属Fusarium真菌为主要种群,而健康植株根际土壤中火丝菌属Pyronema和被孢霉属Mortierella等真菌类群的相对丰度显著高于发病植株根际土壤,尖孢镰孢菌F.oxysporum和刺座霉属的Volutellacolletotrichoides的相对丰度则显著低于发病植株。

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