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菌物学报  2015 

不同碳源和氮源对金针菇降解木质纤维素酶活性的影响

DOI: 10.13346/j.mycosystema.150060, PP. 761-771

Keywords: 羧甲基纤维素酶,木聚糖酶,漆酶,液体发酵

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Abstract:

以3株栽培的金针菇Flammulinavelutipes为材料,研究它们在玉米芯和棉子壳以及不同碳源、氮源培养条件下纤维素、半纤维素和木质素降解酶活性的规律。结果表明,不同金针菇菌株的羧甲基纤维素酶、木聚糖酶和漆酶活力显著不同(P<0.001),同时,培养条件对羧甲基纤维素酶、木聚糖酶和漆酶的活力都有显著影响(P<0.001)。在简单碳源存在的条件下,金针菇的羧甲基纤维素酶和木聚糖酶活力远远低于复杂碳源培养基(P<0.05)。全营养培养基上生长的金针菇的羧甲基纤维素酶和木聚糖酶活力低于缺乏碳源和氮源的培养基(P<0.05)。漆酶活力在无简单氮源培养基上低于全培养基(P<0.05)和无葡萄糖培养基(P<0.05),即复杂碳源和氮源培养基上的漆酶活力低于简单碳源和氮源培养基(P<0.05)。

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