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菌物学报  2015 

与草菇原基形成相关的过氧化物酶基因VvDyP的结构与表达分析

DOI: 10.13346/j.mycosystema.150050, PP. 734-741

Keywords: DyP-type,过氧化物酶,草菇,原基形成,系统发育分析,差异表达

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Abstract:

DyP-type过氧化物酶作为氧化物酶家族中的一员,参与了菌体氧化应激调节反应以及基质降解等过程。本研究从草菇基因组中获得一个DyP-type过氧化物酶的编码基因,将其命名为VvDyP。对该基因进行结构分析,结果显示草菇的DyP-type过氧化物酶编码基因全长为2333bp,含有8个外显子,7个内含子;开放阅读框长为1485bp,编码494个氨基酸。通过系统发育分析发现它与灰盖鬼伞以及糙皮侧耳DyP蛋白同源性最高;分析DyP-type过氧化物酶编码基因在草菇各个时期的表达谱情况并进行荧光定量PCR实验验证发现,草菇的DyP-type过氧化物酶编码基因只在原基中高表达,推测DyP-type过氧化物酶编码基因可以清除过量的活性氧自由基以保证原基的正常形成。

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