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菌物学报  2015 

洁丽香菇胞外粗多糖对小鼠移植性S180肉瘤的抑制作用和免疫功能调节

DOI: 10.13346/j.mycosystema.150043, PP. 779-786

Keywords: 洁丽香菇,胞外多糖,发酵,抑瘤率,细胞因子

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Abstract:

对洁丽香菇胞外粗多糖中多糖和蛋白含量进行测定,其中多糖含量为21.87%,蛋白含量为7.14%。通过体内实验研究该多糖对S180肉瘤的生长抑制作用。结果表明洁丽香菇胞外粗多糖高、中、低剂量组均能不同程度抑制S180肉瘤的生长,其中高剂量组(500mg/kg)抑瘤率最高(为39.44%),同时能显著增加胸腺指数,降低脾指数,有效改善免疫器官受损现象;中、低剂量(250mg/kg,125mg/kg)亦能抑制肿瘤生长,抑瘤率分别为30.43%和23.40%。实验中采用ELISA法对血清中各种细胞因子含量进行测定,结果表明洁丽香菇胞外粗多糖高剂量组可明显诱导血清中TNF-α、IL-12的分泌,低剂量组可诱导IFN-γ、IL-10的分泌。由此认为洁丽香菇胞外粗多糖通过恢复免疫器官功能,增加血清中TNF-α、IL-12、IFN-γ和IL-10的含量,从而达到增强细胞免疫的作用,发挥抗肿瘤活性。

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