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菌物学报  2015 

白灵侧耳栽培种质对高温胁迫的反应研究

DOI: 10.13346/j.mycosystema.150053, PP. 640-646

Keywords: 白灵侧耳,高温胁迫,脂质过氧化,蛋白质羰基,菌丝显微形态

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Abstract:

本文以“中农一号”、“华杂13”、“中农翅鲍”和“CCMSSC00488”4个白灵侧耳菌株为材料,通过测定高温胁迫下菌丝体内硫代巴比妥酸反应物(thiobarbituricacid-reactivesubstances,TBARS)和蛋白质羰基(proteincarbonyl,PCO)含量,观察适温培养和高温胁迫后恢复培养时菌落的生长速率、生长势和菌丝形态特征,研究不同白灵侧耳栽培种质对高温胁迫的反应。研究表明,白灵侧耳不同栽培种质对高温胁迫的反应在氧化损伤程度、菌落形态和菌丝形态特征上都有变化,不同材料的高温伤害程度有着较显著的差异。4个材料高温胁迫条件下TBARS和PCO含量均显著升高,但是升高程度不同,按照TBARS和PCO含量多少排序,依次为CCMSSC00488>华杂13>中农翅鲍>中农一号。高温胁迫处理后恢复生长需要的时间依次为:CCMSSC00488>华杂13>中农翅鲍、中农一号;菌落生长势:中农一号>中农翅鲍=华杂13>CCMSSC00488;菌丝形态特征:菌丝表面增长率、菌丝顶端细胞表面积、菌丝直径和菌丝体分支频率均显著降低,但降低程度以中农一号为最大。菌丝体分支频率与菌落生长势相对应。

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