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菌物学报  2015 

金针菇单、双核菌丝差异表达基因分析

DOI: 10.13346/j.mycosystema.150054, PP. 683-693

Keywords: 转录组,差异表达基因,GO功能,Pathway分析

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Abstract:

对金针菇Flammulinavelutipes单核菌丝W23的菌丝体以及与L11质配后的双核菌丝H1123菌丝体进行了转录组测序,以本实验室已获得的W23基因组为参考基因组研究两样本间差异基因,并对这些差异基因进行了GO功能和Pathway显著性富集分析。差异基因分析显示,两个样本中共有显著性差异表达的基因3504个,其中在双核菌丝中上调、下调的基因数分别为2151和1353个。研究发现差异表达基因含有很多的转录因子基因、蛋白激酶以及WD40repeat-like蛋白。GeneOntology(GO)功能分析结果表明,extracellularregion和membrane-enclosedlumen条目下的差异基因全部为上调表达,而envelope下的差异基因全部为下调表达,以利于双核菌丝分裂时锁状联合的形成而便于核的迁移。Pathway功能富集分析结果表明,脂肪酸、氨基酸以及大部分糖类合成相关基因具有比较活跃的上调表达。说明双核菌丝主要进行营养物质的富集,为下一步在合适条件下分化成原基,进入生殖生长阶段储备物质基础。

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