全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
菌物学报  2015 

侧耳属食用菌高温胁迫条件优化研究

DOI: 10.13346/j.mycosystema.150084, PP. 662-669

Keywords: 糙皮侧耳,白黄侧耳,高温胁迫,脂质过氧化

Full-Text   Cite this paper   Add to My Lib

Abstract:

侧耳属真菌是我国最大规模栽培的食用菌,主要是糙皮侧耳Pleurotusostreatus和白黄侧耳P.cornucopiae。高温是影响侧耳产量和质量的重要因子之一,确定高温胁迫条件是开展高温胁迫机制研究的方法学基础。以糙皮侧耳和白黄侧耳的11个菌株为材料,以硫代巴比妥酸反应产物(thiobarbituricacidreactivesubstances,TBARS)含量为主要生理指标,结合菌丝形态和生长等微生物学参数,开展了双核体菌丝高温胁迫条件的研究,包括胁迫的适宜温度、时间和菌龄等。结果表明,所有供试材料,高温胁迫后菌丝体内TBARS含量在28–40℃、0–48h范围内与温度和时间呈正相关,与之后的恢复生长率呈负相关,高温胁迫后菌丝体内TBARS含量和之后的恢复生长能力均与自身菌丝体生长和子实体形成的适宜温度无关。TBARS含量可作为菌丝高温胁迫生理研究条件确定的指标。与植物的最适温度加10℃不同,基于TBARS含量的糙皮侧耳和白黄侧耳高温胁迫研究条件应为DifcoTMPotatoDextroseAgar培养基,菌丝培养3d,最适温度加12℃,高温胁迫处理48h。

References

[1]  Abrashev R, Stoitsova S, Krumova E, Pashova S, Paunova-Krasteva T, Vassilev S, Dolashka-Angelova P, Angelova M, 2014. Temperature-stress tolerance of the fungal strain Aspergillus niger 26: physiological and ultrastructural changes. World Journal of Microbiology & Biotechnology, 30(5): 1661-1668 [本文引用:1]
[2]  Borsani O, Valpuesta V, Botella MA, 2001. Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiology, 126(3): 1024-1030 [本文引用:1]
[3]  Dai YC, Zhou LW, Yang ZL, Wen HA, Bau T, Li TH, 2010. A revised checklist of edible fungi in China. Mycosystema, 29(1): 1-21 (in Chinese) [本文引用:1]
[4]  Ferreira AS, Totola MR, Borges AC, 2007. Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress. Journal of Thermal Biology, 32(1): 34-41 [本文引用:1]
[5]  Guo XL, Liu ZH, Zhao HW, Xu J, Li HC, 2014. Gene expression of heat-shock proteins and antioxidant enzymes under heat acclimation and heat stress in winter wheat. Acta Agriculturae Boreali-Sinica, 29(4): 13-18 (in Chinese) [本文引用:1]
[6]  Hamid MI, Zeng F, Cheng J, Jiang D, Fu Y, 2013. Disruption of heat shock factor 1 reduces the formation of conidia and thermotolerance in the mycoparasitic fungus Coniothyrium minitans. Fungal Genetics and Biology, 53: 42-49 [本文引用:1]
[7]  Huang BF, 2008. Temperature influences the differential expression of intracellular and extracellular proteins of edible fungus Pleurotus tuber-regium (Fr. ) Sing. Master Thesis, Fujian Agricultural and Forestry University, Fuzhou. 3-38 (in Chinese) [本文引用:1]
[8]  Kong WW, Huang CY, Chen Q, Zou YJ, Zhao MR, Zhang JX, 2012. Nitric oxide is involved in the regulation of trehalose accumulation under heat stress in Pleurotus eryngii var. tuoliensis. Biotechnology Letters, 34(10): 1915-1919 [本文引用:1]
[9]  Li PP, Cheng GF, Zhang JH, Li BB, Xie XJ, Yang SB, 2010. Effects of high temperature stress on physiological characteristics of rice during heading and flowering period. Journal of Jiangsu University-Natural Science Edition, 31(2): 125-130 (in Chinese) [本文引用:1]
[10]  Liu XM, Huang CY, Chen Q, Wu XL, Zhang JX, 2013 a. Study on the metabolic pathway of trehalose in Pleurotus pulmonarius during heat stress recovery. Scientia Agricultura Sinica, 46(24): 5188-5195 (in Chinese) [本文引用:1]
[11]  Liu XM, Huang CY, Chen Q, Wu XL, Zhang JX, 2013 b. Alleviative effects of exogenous trehalose on oxidative damage metabolism in Pleurotus pulmonarius under heat stress. Acta Horticulturae Sinica, 40(8): 1501-1508 (in Chinese) [本文引用:1]
[12]  Lu ZM, Xu Z, Wang K, Chen C, Chencen LL, Song SY, 2009. Differential proteomics studies on thermotolerance of Agaricus bisporus. Journal of Xiamen University (Natural Science), 48(4): 590-593 (in Chinese) [本文引用:1]
[13]  Moller IM, Jensen PE, Hansson A, 2007. Oxidative modifications to cellular components in plants. Annual Review of Plant Biology, 58: 459-481 [本文引用:1]
[14]  Sakamoto Y, Ando A, Tamai Y, 2001. Differential protein expression in the fruiting dikaryon and the non-fruiting monokaryon of Flammulina velutipes. Mycological Research, 5(2): 177-182 [本文引用:1]
[15]  Sekmen AH, Ozgur R, Uzilday B, Turkan I, 2014. Reactive oxygen species scavenging capacities of cotton (Gossypium hirsutum) cultivars under combined drought and heat induced oxidative stress. Environmental and Experimental Botany, 99: 141-149 [本文引用:1]
[16]  Song C, Chen Q, Wu XL, Zhang JX, Huang CY, 2014 a. Heat stress induces apoptotic-like cell death in two Pleurotus species. Current Microbiology, 69(5): 611-616 [本文引用:2]
[17]  Song GC, Chen QZ, Tang CM, 2014 b. The effects of high-temperature stress on the germination of pollen grains of upland cotton during square development. Euphytica, 200(2): 175-186 [本文引用:1]
[18]  Soumen B, 2012. An inductive pulse of hydrogen peroxide pretreatment restores redox-homeostasis and oxidative membrane damage under extremes of temperature in two rice cultivars. Plant Growth Regulation, 68(3): 395-410 [本文引用:1]
[19]  Soumen B, 2013. Heat and chilling induced disruption of redox homeostasis and its regulation by hydrogen peroxide in germinating rice seeds (Oryza sativa L. , cultivar Ratna). Physiology and Molecular Biology of Plants, 19(2): 199-207 [本文引用:2]
[20]  Tang X, Master QPW, Le GW, Shi YH, 2012. Effects of heat treatment on structural modification and in vivo antioxidant capacity of soy protein. Nutrition, 28(11/12): 1180-1185 [本文引用:1]
[21]  Wang HX, 2008. Edible mushroom cultivation. China Agricultural University Press, Beijing. 1-340(in Chinese) [本文引用:1]
[22]  Zheng SY, Zhang JX, Wang HX, 2003. Polyphasic taxonomy of the closely related cultured Pleurotus species in China. Edible Fungi of China, 22(3): 3-6 (in Chinese) [本文引用:1]
[23]  戴玉成, 周丽伟, 杨祝良, 文华安, 图力古尔, 李泰辉, 2010. 中国食用菌名录. 菌物学报, 29(1): 1-21 [本文引用:1]
[24]  郭秀林, 刘子会, 赵会嶶, 徐婧, 李慧聪, 2014. 热锻炼和高温胁迫下冬小麦热激蛋白与抗氧化酶基因表达. 华北农学报, 29(4): 13-18 [本文引用:1]
[25]  黄碧芳, 2008. 不同温度对虎奶菇胞内胞外蛋白差异表达的影响. 福建农林大学硕士论文, 福州. 3-38 [本文引用:1]
[26]  李萍萍, 程高峰, 张佳华, 李秉柏, 谢晓金, 杨沈斌, 2010. 高温对水稻抽穗扬花期生理特性的影响. 江苏大学学报(自然科学版), 31(2): 125-130 [本文引用:1]
[27]  刘秀明, 黄晨阳, 陈强, 邬向丽, 张金霞, 2013 a. 肺形侧耳高温后恢复期间海藻糖代谢途径研究. 中国农业科学, 46(24): 5188-5195 [本文引用:2]
[28]  刘秀明, 黄晨阳, 陈强, 邬向丽, 张金霞, 2013 b. 外源海藻糖对高温胁迫下肺形侧耳氧化损伤的缓解效应. 园艺学报, 40(8): 1501-1508 [本文引用:1]
[29]  陆兆明, 徐祯, 王珂, 陈澄, 陈岑渌璐, 宋思扬, 2009. 双孢蘑菇耐温差异蛋白质组学研究. 厦门大学学报(自然科学版), 48(4): 590-593 [本文引用:2]
[30]  王贺祥, 2008. 食用菌栽培学. 北京: 中国农业大学出版社. 1-340 [本文引用:1]
[31]  郑素月, 张金霞, 王贺祥, 2003. 我国栽培平菇近缘种的多相分类. 中国食用菌, 22(3): 3-6 [本文引用:1]

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133