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红色中缢虫赤潮中浮游植物细胞碱性磷酸酶的研究

DOI: 10.13634/j.cnki.mes20150516, PP. 736-742

Keywords: 浮游植物,ELF,碱性磷酸酶

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Abstract:

2009年12月14日,在对球形棕囊藻Phaeocystisglobosa赤潮的跟踪调查过程中发现一次红色中缢虫赤潮,设置3个站位对红色中缢虫赤潮进行调查监测。利用酶标记荧光(enzymelabeledfluorescence,ELF@97)方法,对浮游植物碱性磷酸酶进行标记,研究红色中缢虫赤潮发生时,单个浮游植物碱性磷酸酶活性特征。得出以下结论:(1)浮游植物碱性磷酸酶总体的标记率达51%,其中甲藻的碱性磷酸酶具有很强的表达,标记率超过99%;硅藻的碱性磷酸酶表达很弱,标记率仅为3.4%。(2)甲藻比硅藻很容易被诱导产生碱性磷酸酶。(3)浮游植物在与红色中缢虫营养盐竞争中处于劣势。

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