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化工学报  2014 

自产气气提与纤维床反应器耦合发酵生产丁醇

DOI: 10.3969/j.issn.0438-1157.2014.05.037, PP. 1821-1827

Keywords: 丁醇,气提,原位分离,发酵,纤维床反应器

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Abstract:

采用发酵产物中的二氧化碳(CO2)和氢气(H2)作为循环气提气源,对丙酮丁醇梭菌(ClostridiumacetobutylicumCGMCC5234)发酵产物进行原位气提,实现丙酮、丁醇和乙醇混合物(ABE)的连续纤维床固定化发酵生产。连续发酵实验进行了12批次共309h,总溶剂ABE当量浓度为133.3g·L-1(其中丁醇83.5g·L-1,丙酮38.4g·L-1,乙醇11.4g·L-1),葡萄糖消耗率为1.29g·(L·h)-1,总溶剂ABE产率为0.431g·(L·h)-1,转化率为0.333g·g-1,其中丁醇产率为0.270g·(L·h)-1,转化率为0.209g·g-1,发酵液中丁醇浓度控制在8~12g·L-1,显著优于游离发酵的结果。气提提取之后冷凝的ABE溶液出现分层现象,其中丁醇相丁醇浓度高达603.7g·L-1,极大地减缓后续分离提纯的负担。结果表明,自产气循环气提与纤维床固定化耦合连续发酵生产ABE(特别是丁醇)的工艺具有可行性和竞争力。

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