全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
化工学报  2014 

介孔TiO2对不同蛋白的选择性固定化性能

DOI: 10.3969/j.issn.0438-1157.2014.05.027, PP. 1750-1758

Keywords: 介孔TiO2,蛋白质,色谱,停留时间,固定化

Full-Text   Cite this paper   Add to My Lib

Abstract:

通过500℃下焙烧H2Ti2O5制备介孔TiO2-500作为高效液相色谱的固定相填料,测试牛血清蛋白(BSA)、肌红蛋白(myoglobin)、溶菌酶(lysozyme)在不同pH环境下的停留时间分布,结果表明,在pH=4.7,三种蛋白质几乎都固定化,在pH=7.4和11条件下,三种蛋白通过色谱柱的停留时间为溶菌酶>肌红蛋白>牛血清蛋白。三种蛋白在介孔TiO2-500表面的宏观常压吸附量的结果与色谱结果相一致,介孔TiO2-500对于三种蛋白的固定化能力为溶菌酶>肌红蛋白>牛血清蛋白。因此,该介孔TiO2对不同蛋白的显著的固定化性能的差异使其在蛋白质的吸附和分离、固定化酶等领域有着潜在的应用。

References

[1]  Magner E. Immobilisation of enzymes on mesoporous silicate materials [J]. Chemical Society Reviews, 2013, 42: 6213-6222
[2]  Proteins at Interfaces II [M]. American Chemical Society, 1995
[3]  Bayne L, Ulijn R V, Halling P J. Effect of pore size on the performance of immobilised enzymes [J]. Chemical Society Reviews, 2013, 42: 9000-9010
[4]  Lue Y, Lu G, Wang Y, Guo Y, Guo Y, Zhang Z, Wang Y, Liu X. Functionalization of cubic Ia3d mesoporous silica for immobilization of penicillin G acylase [J]. Advanced Functional Materials, 2007, 17: 2160-2166
[5]  Lin Z E, Yang G Y. Chemistry of porous materials: from microporous inorganic - Solids to metal-organic frameworks [J]. Chinese Journal of Structural Chemistry, 2004, 23: 1388-1398
[6]  Diaz J F, Balkus K J. Enzyme immobilization in MCM-41 molecular sieve [J]. Journal of Molecular Catalysis B-Enzymatic, 1996, 2: 115-126
[7]  Takimoto A, Shiomi T, Ino K, Tsunoda T, Kawai A, Mizukami F, Sakaguchi K. Encapsulation of cellulase with mesoporous silica (SBA-15) [J]. Microporous and Mesoporous Materials, 2008, 116: 601-606
[8]  Fujimoto C. Titanium dioxide coated surfaces for capillary electrophoresis and capillary electrochromatography [J]. Electrophoresis, 2002, 23: 2929-2937
[9]  Ghazi I, Segura D, Gómez A, Fernández-Arrojo L, Alcalde M, Yates M, Rojas-Cervantes M L, Plou F J, Ballesteros A. Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides [J]. Journal of Molecular Catalysis B: Enzymatic, 2005, 35: 19-27
[10]  Hernández R, Mendieta Wejebe J, Vazquez Alcantara J, Miranda Ruvalcaba R, Garc?a Serrano L, Trujillo Ferrara J. Immobilization of cytochrome P-450 on MCM-41 with different silicon/aluminum ratios [J]. Microporous and Mesoporous Materials, 2005, 80: 25-31
[11]  Bao N Z, Shen L M, Feng X, Lu X H. High quality and yield in potassium titanate whiskers synthesized by calcination from hydrous titania [J]. Journal of the American Ceramic Society, 2004, 87: 326-330
[12]  He M, Feng X, Lu X H, Ji X Y, Liu C, Bao N Z, Xie J W. Application of an ion-exchange model to the synthesis of fibrous titanate derivatives [J]. Journal of Chemical Engineering of Japan, 2003, 36: 1259-1262
[13]  Lin Huaxiang (林华香), Wang Xuxu (王绪绪), Fu Xianzhi (付贤智). Properties and distribution of the surface hydroxyl groups of TiO2 [J]. Progress in Chemistry(化学进展), 2007, 19: 665-670
[14]  Wang D, Zhao J, Chen B, Zhu C. Lattice vibration fundamentals in nanocrystalline anatase investigated with Raman scattering [J]. Journal of Physics-Condensed Matter, 2008, 20(18): 085212
[15]  Yang Zhuhong(杨祝红), Wang Yanfang (王艳芳), Li Licheng (李力成), Wang Changsong (王昌松), Lu Xiaohua(陆小华). Pore structure modification of mesoporous TiO2 for immobilization of protein [J]. Journal of Nanjing University of Technology(南京工业大学学报), 2012, 34: 7-17
[16]  Si P, Ding S, Yuan J, Lou X W, Kim D H. Hierarchically structured one-dimensional TiO2 for protein immobilization, direct electrochemistry, and mediator-free glucose sensing [J]. ACS Nano, 2011, 5: 7617-7626
[17]  Yu J, Zhao H, Ye L, Yang H, Ku S, Yang N, Xiao N. Effect of surface functionality of magnetic silica nanoparticles on the cellular uptake by glioma cells in vitro [J]. Journal of Materials Chemistry, 2009, 19: 1265-1270
[18]  Tao Z, Toms B B, Goodisman J, Asefa T. Mesoporosity and functional group dependent endocytosis and cytotoxicity of silica nanomaterials [J]. Chemical Research in Toxicology, 2009, 22: 1869-1880
[19]  Yu J H, Ju H X. Preparation of porous titania sol-gel matrix for immobilization of horseradish peroxidase by a vapor deposition method [J]. Analytical Chemistry, 2002, 74: 3579-3583
[20]  Calleja G, Serrano D P, Sanz R, Pizarro P, Garcia A. Study on the synthesis of high-surface-area mesoporous TiO2 in the presence of nonionic surfactants [J]. Industrial & Engineering Chemistry Research, 2004, 43: 2485-2492 Industrial target="_blank">
[21]  Liu W, Wang J G, Li W, Guo X, Lu L, Lu X, Feng X, Liu C, Yang Z. A shortcut for evaluating activities of TiO2 facets: water dissociative chemisorption on TiO2-B (100) and (001) [J]. Physical Chemistry Chemical Physics, 2010, 12: 8721-8727
[22]  Li Q, Wang X, Lu X, Tian H, Jiang H, Lü G, Guo D, Wu C, Chen B. The incorporation of daunorubicin in cancer cells through the use of titanium dioxide whiskers [J]. Biomaterials, 2009, 30: 4708-4715
[23]  Qin Danhua (秦丹华), Yao Zhong (姚忠), Wang Haoqi (王浩琦), Xiao Yifan(肖易凡), Yang Jing (杨敬), Xu Hong (徐虹), Wei Ping(韦萍). Immobilization of GGT on meso-TiO2:preparation and properties [J]. CIESC Journal (化工学报), 2011, 62(2): 378-385
[24]  Chaudhuri A S, Bose S. Isolation of protein from dilute solution by chromatographic method [J]. International Journal of Pharmacy and Engineering(IJPE),2013,1(1):12-16
[25]  Wu Z, Zhao D. Ordered mesoporous materials as adsorbents [J]. Chemical Communications, 2011, 47: 3332-3338
[26]  Wang F, Min Y, Geng X. Fast separations of intact proteins by liquid chromatography [J]. Journal of Separation Science, 2012, 35: 3033-3045
[27]  Katiyar A, Ji L, Smirniotis P, Pinto N G. Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size [J]. Journal of Chromatography A, 2005, 1069: 119-126
[28]  Wang A, Liu M, Wang H, Zhou C, Du Z, Zhu S, Shen S, Ouyang P. Improving enzyme immobilization in mesocellular siliceous foams by microwave irradiation [J]. Journal of Bioscience and Bioengineering, 2008, 106: 286-291
[29]  Hartmann M. Ordered mesoporous materials for bioadsorption and biocatalysis [J]. Chemistry of Materials, 2005, 17: 4577-4593
[30]  Lee C H, Lin T S, Mou C Y. Mesoporous materials for encapsulating enzymes [J]. Nano Today, 2009, 4: 165-179

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133